Application of vdac protein in inhibition of cell growth by sodium oxalate or calcium chloride

A technology of sodium oxalate and calcium chloride, which is applied in antibacterial drugs, chemical instruments and methods, and resistance to media-borne diseases, can solve the problems of timely and accurate earthquake prediction, and achieve the goal of preventing and mitigating natural disasters Effect

Inactive Publication Date: 2019-10-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These three methods are usually combined and complement each other, but still fall short of the goal of timely and accurate earthquake prediction

Method used

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  • Application of vdac protein in inhibition of cell growth by sodium oxalate or calcium chloride
  • Application of vdac protein in inhibition of cell growth by sodium oxalate or calcium chloride
  • Application of vdac protein in inhibition of cell growth by sodium oxalate or calcium chloride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Detection of the inhibition rate of 7 peptides to Escherichia coli MG1655

[0029] Each synthetic peptide was dissolved in sterile water so that the stock solution concentration of the peptide was 20 mM. Then, each peptide was diluted to four final concentrations with four corresponding liquid media to form peptide solutions, which were 40 μM, 80 μM, 160 μM, and 320 μM, respectively. Dilute Escherichia coli to 4*10 5 cfu / ml final concentration. According to Table 2, the bacterial solution and the peptide solution were spotted on a 96-well plate, and three parallel controls were set for each spotting well. According to the culture time of Escherichia coli previously determined, put the 96-well plate with the sample in the incubator at 37°C for 20 hours and measure the OD value at 492nm with a microplate reader. According to the measured OD value, the antibacterial rate of various peptides against Escherichia coli in different concentrations and different med...

Embodiment 2

[0035] Example 2 Detection of the inhibition rate of 8 kinds of peptides to Saccharomyces cerevisiae INVSc1

[0036] Each synthetic peptide was dissolved in sterile water so that the stock solution concentration of the peptide was 20 mM. Then, each peptide was diluted to four final concentrations with four corresponding liquid media to form peptide solutions, which were 40 μM, 80 μM, 160 μM, and 320 μM, respectively. Dilute Saccharomyces cerevisiae to 1*10 3 cfu / ml final concentration. According to Table 3, the bacterial solution and the peptide solution were spotted on a 96-well plate, and three parallel controls were set for each spotting well. According to the previously determined yeast culture time, place the 96-well plate with the sample in a 30°C incubator for 21 hours and measure the OD value at 492nm with a microplate reader. According to the measured OD value, the bacteriostatic rate of various peptides against yeast in different concentrations and different media...

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Abstract

According to the invention, the affinity of VDAC3 protein on oxalic acid or calcium salt is proved by experiments. The calcium concentration can be reduced due to formation of organic acid calcium, such as calcium oxalate and the like, and related phenomena, such as spasm and the like, can be caused by reduction of the calcium concentration and the hard and insoluble organic acid calcium salt. Continental or offshore earthquake can be predicted accurately according to inhibition behavior activities and abnormal phenomena that animals suffer from spasm, refuse food, cannot stand and are easy to capture, water birds refuse to go in the water, and seabirds fear spasm and fly towards the mainland; therefore, the invention also provides application of the VDAC3 polypeptide to earthquake prediction, and a simple, convenient and effective earthquake prediction method is established and has great benefit of preventing and reducing natural hazard in the country and even the world.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, relates to the application of VDAC protein in inhibiting cell growth and earthquake prediction by using oxalic acid or calcium salt. Background technique [0002] There are three subtypes of VDAC in mammals such as humans and mice. The amino acid sequence of the primary structure of animal VDAC proteins has great differences, but the basic amino acids are highly conserved. There are more than 12% basic amino acid residues in the mouse VDAC3 protein. By comparing 50 highly homologous amino acid sequences of VDAC3 from different mammals, it was found that the polymorphism of basic amino acids in VDAC3 has a positive correlation with the ability of animals to perceive earthquakes—the more basic amino acids contained More animals are more sensitive to earthquake perception. [0003] The voltage dependence of VDAC is manifested in that the switching state of the channel is controlled...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07K14/47A61P31/04A61P31/10A01K67/027
CPCA01K67/027A01K2207/10C07K14/47Y02A50/30
Inventor刘秋云何建国翁少萍维托·德·平托杜悦邹俊彤李艺祺陈丽贺竹梅徐棒高晔周文良程度李志宁
OwnerSUN YAT SEN UNIV