Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A culture medium and method for tissue culture of Chinese rose

A technology of tissue culture and culture medium, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of low explant proliferation coefficient and rooting rate of induced differentiation rate, and achieve high induced differentiation rate, rapid reproductive effect

Inactive Publication Date: 2018-08-28
河南红枫生物高科股份有限公司 +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the induction differentiation rate, explant proliferation coefficient and rooting rate of Chinese rose tissue culture were low using the above-mentioned differentiation, subculture and rooting medium.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Growth environment

[0058] (1) MS solid medium was used, in which the mass concentration of agar was 7g / L, the mass concentration of sucrose was 30g / L, and the pH value was 5.8. ).

[0059] (2) Tissue culture room, light time 12h / d, light intensity 3500lx, culture temperature 25°C, relative humidity 65%, sterilized by ultraviolet light for 15min every 3d.

[0060] When the MS medium is sterilized by high pressure steam, the temperature is 126°C for 5 minutes, which is different from the traditional 121°C for 30 minutes. After the medium sterilized by this method was cultivated in a light incubator (38° C., without light) for 7 days, no colonies were found. It is proved that under such conditions, the sterilization effect can be achieved completely. The power of the sterilizing pot used in the test is 2kw, which saves 0.5h of time compared with the traditional method, and saves 1 kilowatt-hour of electricity for each sterilization.

[0061] 2. The collection of e...

Embodiment 2

[0101] 1. Growth environment

[0102] (1) MS solid medium was used, in which the mass concentration of agar was 7g / L, the mass concentration of sucrose was 30g / L, and the pH value was 5.8. ).

[0103] (2) Tissue culture room, light time 12h / d, light intensity 3500lx, culture temperature 25°C, relative humidity 65%, sterilized by ultraviolet light for 15min every 3d.

[0104] When the MS medium is sterilized by high pressure steam, the temperature is 126°C for 5 minutes, which is different from the traditional 121°C for 30 minutes. After the medium sterilized by this method was cultivated in a light incubator (38° C., without light) for 7 days, no colonies were found. It is proved that under such conditions, the sterilization effect can be achieved completely. The power of the sterilizing pot used in the test is 2kw, which saves 0.5h of time compared with the traditional method, and saves 1 kilowatt-hour of electricity for each sterilization.

[0105] 2. The collection of e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of tissue culture, and in particular to a culture medium and a method for tissue culture of grandiflora roses. The culture medium for tissue culture of grandiflora roses comprises a differential medium, a subculture medium and a rooting medium, wherein the differential medium is an MS (Murashige and Skoog) culture medium comprising 0.5-2.0mg / L of 6-BA (6-benzylamino adenine), 0.1mg / L of NAA (1-naphthylacetic acid) and 0.1-0.3mg / L of TDZ (thidiazuron); the subculture medium is an MS culture medium comprising 1.0mg / L of 6-BA and 0.1-0.5mg / L of NAA, the rooting medium is an MS culture medium comprising 0.2-1.0mg / L of NAA. By adopting the culture medium provided by the invention, the induced differentiation rate, the explant proliferation coefficient and the rooting rate of the grandiflora roses can be remarkably increased.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of Chinese roses. Background technique [0002] Large-flowered rose, also known as perfume rose, strong-flowered rose, and ornamental rose, is a plant of the genus Rosa. It is suitable for growing in a sunny and air-ventilated environment, and it has no strict requirements on the soil. There are many varieties, which are the main part of modern rose. Its characteristics are: strong plants, single or group flowers, large flowers, elegant and beautiful flower shapes, many colors, bright and lively, aromatic smell, and strong ornamental value. The main production area is Nanyang City, Henan Province, and it is also produced in Shandong, Yunnan, Jiangsu, Beijing, Shanghai and other places. [0003] The more common methods of propagation of large-flowered roses are grafting and cuttings. The advantage of grafted rose seedlings is that...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丹张茂孟伟芳赵志汝王东超
Owner 河南红枫生物高科股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com