Pectinase preparation and application thereof

Abstract

The invention discloses a pectinase preparation and an application thereof and belongs to the field of enzyme preparations. The thermal stability of pectinase at 60 DEG C is remarkably improved by using glycine, Zn<2+> and alanine, and the residual activity of the pectinase is higher than 50% after the pectinase is kept at the constant temperature of 60 DEG C for 10 h, so that the pectinase is better applicable to surface finishing of cotton fabric.

Description

technical field [0001] The invention relates to a pectinase preparation and application thereof, belonging to the field of enzyme preparations. Background technique [0002] Alkaline pectinase (E.C.4.2.2.2, PGL for short), the full name of polygalacturonate lyase, the enzyme widely exists in bacteria, yeast, fungi, plants and some parasitic nematodes. Alkaline pectinase is widely used in various fields such as food, textile, papermaking, environment, and biotechnology, and plays a huge role in tea and coffee fermentation, textile and plant fiber processing, oil extraction, and pectin-containing industrial wastewater treatment. Alkaline pectinase has high activity under alkaline conditions, and can use trans-elimination to cut the α-1,4-glycosidic bond of polylacturonic acid, and decompose pectin into unsaturated oligogalacturonic acid . [0003] There are many accompanying impurities on the outermost surface of the primary cell wall of cotton fiber, such as: pectin, wax, n...

AI Technical Summary

Problems solved by technology

The traditional chemical textile refining method is the hot alkali method, which consumes a lot of chemicals and water resources, causing serious environmental pollution. According to statistics, about 70% of the wastewater in the printing and dyeing process is produced in this step

In addition, hot alkali treatment will cause serious damage to the fiber, and the mechanical strength will be greatly reduced