Dimeric carpienyne a and its application in the preparation of drugs for the prevention and treatment of neurodegenerative diseases

A dimerized carbopolene, neurodegenerative technology, applied in nervous system diseases, drug combinations, pharmaceutical formulations, etc., can solve the problems of multiple donor sites and limited sources of autologous nerves

Active Publication Date: 2019-07-30
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, autologous nerve transplantation is still the standard operation for solving nerve defects in the clinical treatment process, but due to the limited source of autologous nerves and many complications at the donor site

Method used

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  • Dimeric carpienyne a and its application in the preparation of drugs for the prevention and treatment of neurodegenerative diseases
  • Dimeric carpienyne a and its application in the preparation of drugs for the prevention and treatment of neurodegenerative diseases
  • Dimeric carpienyne a and its application in the preparation of drugs for the prevention and treatment of neurodegenerative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 A large amount of fermentation of the fungus 71-10-1-1 of the genus Charcoa and its sample pretreatment method

[0026] (1) The fungus 71-10-1-1 of the genus Anthracum was activated on a PDA slant and then inoculated in PDB medium, at 25°C at 200r.min -1 Shake culture for 5 days to prepare seed solution, then inoculate 20 Erlenmeyer flasks with rice culture medium according to 5% inoculation amount, and culture at 25°C for 49 days to obtain fermented product. The rice medium is composed of the following components: rice 70g / bottle, purified water 105L / bottle.

[0027] (2) The fermented product was added to ethyl acetate for soaking and extraction three times, and the extract was concentrated to dryness under reduced pressure to obtain a crude extract (43.2 g).

Embodiment 2 2

[0028] The preparation of embodiment 2 dipoly carpienyne A

[0029] The crude extract was passed through a silica gel column and eluted with cyclohexane and methanol to obtain cyclohexane part C (12.5g) and methanol part w (21.9g); then the methanol part M was subjected to medium and low pressure ODS column chromatography, followed by Four fractions (w1, w2, w3, w4) were obtained by methanol-water gradient elution with a volume ratio of 20:80, 50:50, 70:30 and 100:0; The obtained sub-fraction w3 (3.1g) was subjected to medium and low pressure liquid phase ODS column chromatography, followed by volume ratios of 20:80, 30:70, 40:60, 45:55, 50:50, 55:45, 60: 40, 100:0 methanol-water gradient elution, w3-1, w3-2, w3-3, w3-4, w3-5, w3-6, w3-7, w3-8 and w3-9 were obtained 9 subfractions. The sub-fraction w3-9 (1.5 g) obtained by eluting with methanol-water in a volume ratio of 100:0 was subjected to silica gel column chromatography, and the volume ratios were 100:0, 97:3, 95:5, 93...

Embodiment 3 2

[0035] Example 3 Dipolymer carpienyne A improves the learning and memory ability of senile dementia Drosophila and its activity test method

[0036] (1) Cultivation of Alzheimer's Drosophila

[0037] w 1118 (isoCJ1) was used as the control group of the experiment, the background Drosophila, abbreviated as "2U". Successful transfer into pathogenic Aβ 42 The Drosophila protein is (UAS-Aβ 42 ; abbreviated as "h29.3"). This strain of Drosophila was crossed with Drosophila expressing the Gal4 promoter in the whole brain to obtain elav-GAL4 c155 (P35) and Aβ 42 Drosophila strains.

[0038] (2) Administration of Alzheimer's Drosophila

[0039] Three groups were set up in the experiment: healthy fruit flies without drug control, diseased fruit flies without drug control and diseased fruit flies with administration.

[0040] The parents of all tested fruit flies were reared and bred in a fly house with a constant temperature of 24° C. and a constant humidity of 42% RH (Relative...

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Abstract

The invention belongs to the field of natural medicine and, particularly, relates to meroterpenoid compound bis-poly(Biscogniauxia-enyne) A and application thereof. The bis-poly(Biscogniauxia-enyne) A has the structure shown as in formula (I). The invention also provides application of the formula (I) compound in the preparation of drugs for preventing or treating neurodegenerative diseases.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to dimeric carpienyne A and its use for preventing and / or treating neurodegenerative diseases. Background technique [0002] Alzheimer's disease is a kind of central nervous system degenerative disease syndrome caused by brain diseases, characterized by progressive cognitive dysfunction and memory impairment, which manifests as intelligence (including memory, learning ability, direction recognition ability, language ability, etc.) ability, comprehension, and judgment). Alzheimer's disease (Alzheimer's disease, AD), vascular dementia (VA), Lewy body dementia (Dementia with Lewy bodies, DLB) and frontotemporal dementia (Frontotemporal dementia, FTD) are generally common )Wait. Among all dementia patients, Alzheimer's disease patients account for 50-70%, which is the most common type of senile dementia. [0003] The drugs currently on the market for the treatment of Alzh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61P25/28A61P25/16
CPCA61K31/352
Inventor 高昊陈国栋姚新生赵欢郭良栋胡丹王传喜秦盛莹邹剑
Owner JINAN UNIVERSITY
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