Flavivirus human monoclonal antibody and application

A technology of antibodies and antigens, applied in the field of medicine, can solve the problems of unknown affinity, poor specificity, limited antibody use and sensitivity, etc., and achieve the effect of huge clinical detection and basic research application value
CN106589116AActive Publication Date: 2017-04-26INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2017-04-26

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Abstract

The invention discloses a flavivirus human monoclonal antibody and application, which belong to the technical field of medicine. According to the flavivirus human monoclonal antibody and the application provided by the invention, three antibodies capable of protein-binding with ZIKV-E are acquired, and binding sites of the three antibodies are confirmed. The three antibodies are completely different from a reported ZIKV antibody sequence, and are three new-found antibodies. The binding constants of the three antibodies and the ZIKV-E are 39.9pM (Z5), 44.7pM (Z6) and 200pM (Z7), which show that the three human monoclonal antibodies have a higher ZIKV-E protein-binding capacity. Through competiton experiments, the binding sites of the three antibodies have competitive relationships with 2A10G6, and the phenomenon shows that the binding sites of the three antibodies are near FL, and E protein of flaviviridae is highly conserved at the FL part. The antibody provided by the invention effectively detects the infection of common flaviviridae viruses: ZIKV, dengue 1-4 type and yellow fever viruses, and has huge application values on clinic detection and fundamental research.
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Description

technical field

[0001] The invention relates to a flavivirus human monoclonal antibody and its application, belonging to the technical field of medicine. Background technique

[0002] The Zika outbreak in Central and South America in 2015 has infected millions of people in 69 countries, and several imported cases have also appeared in my country. With the flow of population and climate change, there is a high risk of Zika virus epidemic in my country. However, due to Zika virus infection, 80% of patients have no obvious symptoms, and 20% of patients have mild symptoms, which will not cause patients to pay attention. Zika virus (ZIKV) belongs to the Flaviviridae family and is mainly transmitted by mosquito bites. At the same time, Zika virus can pass through the mother-fetal barrier and cause microcephaly in newborns. In addition, Zika virus can be transmitted sexually. Clinical data show that Zika virus can be transmitted to women through male patients, and can also be t...

Claims

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