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Compound Chinese herbal medicinal preparation for preventing and curing white spot disease of procambarus clarkia and method thereof

A technology of white spot syndrome and Procambarus clarkii, applied in medical preparations containing active ingredients, applications, antiviral agents, etc., can solve difficult problems such as increasing production, reducing disease mortality, and simple operation Effect

Inactive Publication Date: 2017-05-10
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cultivating shrimp seedlings that do not carry WSD is a way to prevent and control WSD. However, judging from the current high virus carrier rate of shrimp, it is very difficult to obtain broodstock that do not carry WSD
In addition, even though shrimp seedlings that do not carry WSD have been bred, it is still difficult to kill the virus remaining in the water and the environment in ponds where WSD disease has already broken out

Method used

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  • Compound Chinese herbal medicinal preparation for preventing and curing white spot disease of procambarus clarkia and method thereof
  • Compound Chinese herbal medicinal preparation for preventing and curing white spot disease of procambarus clarkia and method thereof
  • Compound Chinese herbal medicinal preparation for preventing and curing white spot disease of procambarus clarkia and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Materials and location

[0022] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0023] In this example, a lobster farm in Hefei was selected as the experimental site. After investigation, the main species of shrimp cultured in this farm was Procambarus clarkii, and last year (2015) there was a phenomenon of mass disease.

[0024] 2. Method

[0025] 2.1 Preparation of compound Chinese herbal medicine preparation

[0026] Isatis root, rhubarb, Houttuynia cordata and allicin were dried and superfinely pulverized to obtain the raw material powder. By weight, the raw material powder was divided into 10 parts of Radix isatidis, 12 parts of rhubarb, 12 parts of Houttuynia cordata and 15 parts of allicin. Mix to obtain the compound Chinese herbal medicine preparation.

[0027] 2.2 Preparatio...

Embodiment 2

[0037] 1. Materials and location

[0038] With embodiment 1.

[0039] 2. Method

[0040] 2.1 Preparation of compound Chinese herbal medicine preparation

[0041] With embodiment 1.

[0042] 2.2 Preparation of medicated feed

[0043] The compound Chinese herbal medicine preparation is added into the feed for Procambarus clarkii in an amount of 0.2% by weight, and mixed evenly to make a drug feed with a particle size of 1.5 mm for standby use.

[0044] 2.3 Prevention methods and results

[0045] Divide the Procambarus clarkii into 5 groups. From mid-April 2016, feed the Procambarus clarkii in groups 1-4 continuously for 20, 30, 40, and 50 days with the prepared drug feed, and the fifth group is used as the control group. , fed with conventional feed. In the last ten days of July, 2016, the cumulative mortality of Procambarus clarkii was counted, and the cumulative mortality of the experimental group was compared with the cumulative mortality of the control group, and the c...

Embodiment 3

[0049] 1. Materials and location

[0050] With embodiment 1.

[0051] 2. Method

[0052] 2.1 Preparation of compound microecological feed

[0053] The special feed for Procambarus clarkii is purchased in the market, and the particle size of the special feed is 1.5 mm, and the protein content is 24% (mass ratio). In the special feed, add probiotics (the probiotics are composed of photosynthetic bacteria and bacillus in equal parts), and the addition ratio is: add 1 × 10 respectively in every gram of special feed. 4 CFU, 5×10 4 CFU, 1×10 5 CFU, 5×10 5 CFU, 1×10 6 For the probiotics of CFU, 5 kinds of probiotics feeds were obtained for standby use.

[0054] 2.2 Prevention and control steps

[0055] The Procambarus clarkii was further divided into 6 groups, the 1st to 5th groups were the experimental group, and the 6th group was the control group.

[0056] In mid-April 2016, the Procambarus clarkii in the experimental group was continuously fed for 30 days with the probio...

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Abstract

The present invention discloses a compound Chinese herbal medicine preparation for preventing and curing white spot disease (WSD) of procambarus clarkia and a method thereof. The compound Chinese herbal medicine preparation comprises the following components: radix isatidis, rheum officinale, herba houttuyniae and allicin. The method comprises the following steps: the compound Chinese herbal medicinal preparation is mixed with feed and the feed is continuously used to feed for 30-40 days before a season of an onset of the WSD of the procambarus clarkia. Compared with the prior art, especially for ponds which the WSD outbreak, the provided compound Chinese herbal medicine preparation for preventing and curing the WSD of the procambarus clarkia can control the incidence of the WSD of the procambarus clarkia to a very low level, the method is simple in operations, and the preparation has very important meanings for preventing and curing the WSD of the procambarus clarkia, reducing morbidity of the procambarus clarkia caused by the WSD, and improving yields.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a compound Chinese herbal medicine preparation for preventing and treating white spot syndrome of Procambarus clarkii and a method thereof. Background technique [0002] In recent years, white spot syndrome (White Spot Disease, WSD) has gradually become an important disease of farmed shrimp. The International Organization for Animal Health (OIE) lists WSD as a must-report disease, and the Ministry of Agriculture lists it as a first-class animal disease. Shrimp is prone to outbreaks of the disease from late April to early July, and the prevailing water temperature is 20-28°C. The climate is characterized by a large temperature difference between day and night on sunny days, continuous sunny days, the temperature rises rapidly, continuous cloudy days and heavy rains, the temperature drops rapidly, the water temperature is prone to drastic changes, and the physical and chemical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/30A23K20/105A23K10/18A01K61/00A61K36/78A61P31/20A61K31/255
Inventor 王永杰陈红莲陈云生徐双贵
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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