Cell incubator and operating method thereof

Abstract

The invention provides a cell incubator, comprising a first box, a second box, a first box door, a temperature measurement device, heating devices, a fan heater and a heat insulation device, wherein the second box is arranged inside the first box, and a sealed spaces is formed between the second box and the first box; the first box door is arranged on the front side part of the first box, and a sealing part is arranged between the first box and the first box door; the temperature measurement device is arranged inside the second box; the heating devices are arranged on the inner side of the first box door and the side part of the second box; the fan heater is arranged in the sealed space; the heat insulation device is arranged at the outer side part of the first box or the inner side part of the first box. The cell incubator has the advantages of good temperature control effect and the like.

Description

technical field [0001] The invention relates to cell culture, in particular to a cell culture box and its working method. Background technique [0002] In the fields of medicine, life health, environment, etc., the cell culture workstation provides a culture environment in an ultra-clean closed space (reaching ISO 5 or GMP level), and the built-in incubator is a special device for culturing cells in a simulated physiological environment in vivo , should have temperature control characteristics such as constant temperature, high temperature uniformity, rapid temperature recovery, and no condensation caused by temperature difference. The current incubator heating methods are: [0003] 1. Water jacket type, the stability of this heating method depends largely on the stability and response time of the heating wire, that is, choosing a reasonable thickness and diameter of the heating wire to slow down the internal consumption of the heating wire elements, and requires irregular ...

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Problems solved by technology

[0003] 1. Water jacket type, the stability of this heating method depends largely on the stability and response time of the heating wire, that is, choosing a reasonable thickness and diameter of the heating wire to slow down the internal consumption of the heating wire elements, and requires irregular heating Filling, cleaning, emptying and monitoring the water tank, which becomes especially difficult for the incubator design

[0004] 2. Air jacket type, this heating method has poor heating uniformity, the air duct and tuyere added to the inner cavity wall of the internal circulation type have high design requirements and high cost, and the structure of the external circulation type is relatively simple, and the thermal conductivity is not good all

[0005] 3. Direct heating, this heating method has high temperature control accuracy, and the six-sided heating method can basically eliminate the condensation water problem of the glass door, but it will cause uneven distribution of the temperature field in the waiting box, destroying the consistency of the cell culture environment, and When one side or more of the inner wall of the incubator fails, it will have a great impact on the temperature control stability of the chamber, especially for the stability and continuity of the cell culture environment caused by the replacement of heating plates and parts. difficulty

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