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Microsatellite DNA (Deoxyribonucleic Acid) marker fingerprint spectrum of red ramies and application of microsatellite DNA marker fingerprint spectrum

A DNA labeling and fingerprinting technology, applied in recombinant DNA technology, DNA/RNA fragments, microbial determination/inspection, etc. question

Inactive Publication Date: 2017-05-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As far as the country is concerned, due to the introduction and utilization of kenaf species between regions in the 20th century, the synonym phenomenon of kenaf varieties is very serious, and the genetic similarity of local varieties is high. It is difficult for traditional morphological and cytological identification methods to accurately and truly identify kenaf. Different species of hemp need to be analyzed and identified at the molecular level

Method used

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  • Microsatellite DNA (Deoxyribonucleic Acid) marker fingerprint spectrum of red ramies and application of microsatellite DNA marker fingerprint spectrum
  • Microsatellite DNA (Deoxyribonucleic Acid) marker fingerprint spectrum of red ramies and application of microsatellite DNA marker fingerprint spectrum
  • Microsatellite DNA (Deoxyribonucleic Acid) marker fingerprint spectrum of red ramies and application of microsatellite DNA marker fingerprint spectrum

Examples

Experimental program
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Embodiment 1

[0036]Example 1 Construction of kenaf microsatellite DNA marker fingerprints: According to the amplification of each material and each primer (64 pairs of SSR primers) in Table 2 above, the DNA fingerprints of 24 kenaf varieties (lines) were drawn. In this DNA fingerprint, each kenaf variety (line) has its specific DNA fingerprint, which can distinguish these 24 varieties (lines) from each other (see figure 1 with Table 2). The names and sources of the 24 varieties (lines) are shown in Table 1.

[0037] SEQUENCE LISTING

[0038] Fujian Agriculture and Forestry University

[0039] A kenaf microsatellite DNA marker fingerprint and its application

[0040] 128

[0041] 128

[0042] PatentIn version 3.3

[0043] 1

[0044] 23

[0045] DNA

[0046] Artificial sequence

[0047] 1

[0048] atatttgttt gatcacaccg tgt 23

[0049] 2

[0050] 20

[0051] DNA

[0052] Artificial sequence

[0053] 2

[0054] ccttatcagc gttcagggca 20

[0055] 3

[0056] 21 ...

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Abstract

The invention relates to a DNA (Deoxyribonucleic Acid) fingerprint spectrum of red ramie germplasm resources. The fingerprint spectrum is a DNA fingerprint spectrum constructed by taking combination of 64 pairs of SSR (Simple Sequence Repeat) primer allelic segments as a foundation and taking 24 red ramie varieties / series as test materials through DNA extracting, PCR (Polymerase Chain Reaction) amplification, identification of a PCR product and data analysis, and finally taking 215 SSR markers. The fingerprint spectrum can be used for distinguishing red ramie varieties / series for testing and can be used for identifying or analyzing the purity of the red ramie varieties / series. Compared with traditional morphological and cytological identification, the fingerprint spectrum has the advantages of short detection time, high accuracy and good repeatability.

Description

technical field [0001] The invention belongs to molecular marker technology, in particular to a kenaf microsatellite DNA marker fingerprint and its application. Background technique [0002] Kenaf ( Hibiscus cannabinus ) is an important natural bast fiber crop in China, mainly grown in China, Thailand and India. The number of chromosomes in cultivated kenaf is 2n=2X=36. Kenaf has the characteristics of drought resistance, salt and alkali resistance, strong stress resistance, well-developed root system, fast growth speed, high biological yield, and easy cultivation. It is widely used in the fields of pollution, feed, sesame oil and medicine. [0003] High-quality kenaf varieties contribute significantly to the yield and quality of kenaf. my country has been planting kenaf since the 1920s, and began to introduce breeding research in the 1970s, and successively launched Fuhong 1, Fuhong 2, Fuhong 952 and Fuhonghang 992, among which Fuhong 952 is famous for its High-yield, h...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 张立武万雪贝祁建民张力岚徐建堂林荔辉
Owner FUJIAN AGRI & FORESTRY UNIV
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