Pet ovarian tissue cryopreservation resuscitation reagent and cryopreservation resuscitation method

A technology of ovarian tissue and reagents, applied in biochemical equipment and methods, tissue culture, preservation of human or animal bodies, etc., can solve problems that have not been developed and applied, achieve high tissue recovery activity, improve damage, and reagent components clear effect

Inactive Publication Date: 2017-05-24
上海尚维生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such technologies in the field of pet heal

Method used

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  • Pet ovarian tissue cryopreservation resuscitation reagent and cryopreservation resuscitation method
  • Pet ovarian tissue cryopreservation resuscitation reagent and cryopreservation resuscitation method
  • Pet ovarian tissue cryopreservation resuscitation reagent and cryopreservation resuscitation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0057] Example 1-5: Preparation of Pet Ovary Tissue Cryopreservation Reagent

[0058] (1) Take the preparation of 100ml cryopreservation reagent 1 as an example: Measure dimethyl sulfoxide (purchased from SIGMA), propylene glycol (purchased from SIGMA), MEM medium (purchased from GIBCO) according to the amounts shown in Table 1 ) into a 250ml blue-mouth bottle (purchased from Shu Niu), shake gently to mix well, and weigh Lycium barbarum polysaccharide (purchased from Snotte Biology), polyvinylpyrrolidone ((abbreviated as PVP) purchased from SIGMA), glucose (purchased from SIGMA ) into the above bottle, shake and mix. After the solids are completely dissolved, use a 0.22 μm filter (purchased from MILLIPORE) to filter and sterilize, filter into a 100ml blue-necked bottle (purchased from Shuniu) that has been sterilized by autoclaving, and store at 4°C for later use;

[0059] (2) Take the preparation of 100ml cryopreservation reagent 2 as an example: Measure dimethyl sulfoxide (...

Embodiment 6-10

[0064] Example 6-10: Preparation of Pet Ovary Tissue Resuscitation Reagent

[0065] (1) Take the preparation of 100ml resuscitation reagent 1 as an example: Measure vitamin E (oily liquid, purity ≥ 98%) (purchased from SIGMA) and MEM medium (purchased from GIBCO) according to the amounts shown in Table 2 Put it into a 250ml blue-necked bottle (purchased from Shu Niu), shake gently to mix well, weigh Lycium barbarum polysaccharide (purchased from Snotte), polyvinylpyrrolidone (purchased from SIGMA), and glucose (purchased from SIGMA) into the above bottle , shake to mix. After the solids are completely dissolved, use a 0.22 μm filter (purchased from MILLIPORE) to filter and sterilize, filter into a 100ml blue-necked bottle (purchased from Shuniu) that has been sterilized by autoclaving, and store at 4°C for later use;

[0066] (2) Take the preparation of 100ml resuscitation reagent 2 as an example: according to the amounts shown in Table 2, measure vitamin E (purchased from SI...

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Abstract

The invention provides a pet ovarian tissue cryopreservation resuscitation reagent which comprises: (1) a cryopreservation resuscitation reagent 1 including 2-7W/V% of lycium barbarum polysaccharide, 6-14V/V% of dimethyl sulfoxide, 2-7V/V% of propylene glycol and 80-90V/V% of an MEM (Memminimum Essential Medium) culture medium; (2) a cryopreservation resuscitation reagent 2 including 8-10W/V% of lycium barbarum polysaccharide, 6-20V/V% of dimethyl sulfoxide, 5-15V/V% of propylene glycol and 70-80V/V% of an MEM culture medium; (3) a cryopreservation resuscitation reagent 3 including 15-20W/V% of lycium barbarum polysaccharide, 6-20V/V% of dimethyl sulfoxide, 5-15V/V% of propylene glycol and 70-80V/V% of an MEM culture medium. The invention further provides a pet ovarian tissue cryopreservation resuscitation reagent group and a pet ovarian tissue cryopreservation and resuscitation method.

Description

technical field [0001] The invention relates to cryopreservation of ovarian tissue, in more detail, the invention relates to a reagent for cryopreservation and recovery of pet ovarian tissue and a method for cryopreservation and recovery of pet ovarian tissue. Background technique [0002] Pets (mainly dogs and cats as companion animals) can accompany humans, relieve the pressure in daily life, delight the mood, promote human health, and become a member of the family. With the development of my country's economy and the aggravation of aging, the demand for pets is increasing. While the number of pets has increased, industries such as pet food, pet supplies, and pet training have developed vigorously. The field of pet health has not received enough attention and has not yet been standardized. Compared with the human health market, the pet health market has a lot of room to be tapped. Caring for pets and paying attention to pet health. [0003] At home and abroad, it is ge...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/071
CPCA01N1/0221A01N1/0284C12N5/0682C12N2500/34C12N2500/38C12N2500/50C12N2501/165
Inventor 朱梅花
Owner 上海尚维生物科技有限公司
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