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Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus

A technology of Bacillus atrophaeus and Lycium barbarum root, which is applied in the direction of application, bacteria, and fungicides, can solve the problems of Bacillus atrophaeus that have not been prevented and controlled of Lycium barbarum root rot, and achieve the effects of inhibiting outbreaks, increasing yield, and reducing morbidity

Active Publication Date: 2017-05-24
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the report that this bacterium is applied to control plant disease aspect is less, and the research involved mainly contains: control bitter gourd gray mold (CN105670964A), control potato beetle larvae (CN104164378A), control cotton boll blight (CN102747005A), control tomato blight , capsicum early blight, cotton blight (CN104531545A), etc., but there is no report of Bacillus atrophaeus for preventing and treating wolfberry root rot

Method used

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  • Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus
  • Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus
  • Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus

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Embodiment 1

[0027] Embodiment 1: Isolation, screening of bacterial strain

[0028] (1) Separation of strains

[0029] Five rhizosphere soil samples of healthy, dying and dead wolfberry trees were collected from Shilaba Village, Zhongning County, Ningxia Province. Weigh 10.0 grams of Lycium barbarum rhizosphere soil, add 90 mL of sterile water for every 10 grams of soil, put it into a pre-prepared conical flask filled with glass beads, place it on a constant temperature shaker at 37°C and shake at 180 rpm for 20-30 minutes. Gradient dilution method, the soil suspension was diluted to 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 different gradients. Draw 0.1 mL of soil solution with appropriate dilution and place it on the LB plate medium, and spread it evenly (in this experiment, 10 -4 、10 -5 、10 -6 Three gradients were applied). Cultivate in a constant temperature incubator at 37°C for 24 hours, and pick a single colony. The obtained strains were purified by the three-section line method ...

Embodiment 2

[0046] Embodiment 2: the preparation of bacterial strain GQJK17 biocontrol agent

[0047] The specific preparation method is as follows:

[0048] The Bacillus atrophaeus GQJK17 that was preserved on the slant and antagonized the root rot of Lycium barbarum was transferred to a test tube containing 5 mL of bean sprout juice liquid medium, and cultured in a constant temperature shaker at 37°C and 180 rpm for 12 hours. Then inoculate into 100mL / 250mL liquid bean sprout juice culture medium according to 1% inoculum amount, put in 37 ℃, 180rpm constant temperature shaker and cultivate 24h, make concentration be 2 * 10 8 cfu / mL bacterial suspension.

[0049] Liquid bean sprout juice culture medium, its raw material composition is: 100g of soybean sprouts, 50g of sucrose, 1000mL of water, natural pH;

[0050] The preparation method is as follows: boil soybean sprouts for 30 minutes, filter with four layers of gauze, add sucrose to make the volume 1000mL.

Embodiment 3

[0051] Embodiment 3: field test

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Abstract

The invention discloses bacillus atrophaeus antagonistic to lycium chinensis root rot with the classification name of bacillus atrophaeus GQJK17, preserved in CGMCC (China General Microbiological Culture Collection Center) of CCCCM (China Committee of Culture Collection for Microorganisms) on November 10, 2016, with the strain preservation number of CGMCC NO.1 3254. The bacillus atrophaeus GQJK17 obtained through separation has a higher antagonistic effect on the fructus lycii root rot, a new biocontrol bacterium is provided for biocontrol of the lycium chinensis root rot, and the type of biocontrol preparations is increased.

Description

technical field [0001] The invention relates to a strain of bacillus atrophaeus antagonizing wolfberry root rot and application thereof, belonging to the field of biotechnology. Background technique [0002] Lycium barbarum is a famous and precious traditional Chinese medicine. Lycium barbarum fruit has high nutritional, health care and medicinal value. It is also known as "four treasures of traditional Chinese medicine" together with Qiongzhen Ganoderma lucidum, Changbai Mountain ginseng and Dong'e Ejiao. With the development of the economy and the progress of the society, people are more and more aware of the value of wolfberry, prompting people to carry out extensive product development of wolfberry, and achieved important results. Ningxia wolfberry has the largest cultivation area in China. With the continuous expansion of wolfberry planting area, the incidence of wolfberry root rot in old production areas and new production areas has increased year after year, and it ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 丁延芹杜秉海马锦锦姚良同汪城墙刘凯
Owner SHANDONG AGRICULTURAL UNIVERSITY
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