Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus

A technology of Bacillus atrophaeus and Lycium barbarum root, which is applied in the direction of application, bacteria, and fungicides, can solve the problems of Bacillus atrophaeus that have not been prevented and controlled of Lycium barbarum root rot, and achieve the effects of inhibiting outbreaks, increasing yield, and reducing morbidity

Active Publication Date: 2017-05-24
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the report that this bacterium is applied to control plant disease aspect is less, and the research involved mainly contains: control bitter gourd gray mold (CN105670964A), control potato beetle larvae (CN104164378A)

Method used

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  • Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus
  • Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus
  • Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1: Isolation and screening of strains

[0028] (1) Separation of strains

[0029] Five healthy, dying and dead rhizosphere soil samples of wolfberry trees were collected from Shilaba Village, Zhongning County, Ningxia Province. Weigh 10.0 g of wolfberry rhizosphere soil, add 90 mL of sterile water per 10 g of soil into a pre-prepared triangular flask containing glass beads, and place it on a constant temperature shaker at 37°C and shake at 180 rpm for 20-30 minutes. Gradient dilution method, dilute the soil suspension to 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 Wait for different gradients. Pipette 0.1mL of the soil solution with a suitable dilution and place it in the LB plate medium, and spread it evenly (this experiment selects 10 -4 , 10 -5 , 10 -6 Three gradients for coating). Incubate for 24 hours in a 37°C constant temperature incubator, and pick a single colony. The obtained strains were purified by a three-zone streaking method until a single colony was obta...

Example Embodiment

[0046] Example 2: Preparation of biocontrol preparation of strain GQJK17

[0047] The specific preparation method is as follows:

[0048] The Bacillus atrophaeus GQJK17, which was preserved on the slant, which antagonizes wolfberry root rot, was transferred to a test tube containing 5 mL of bean sprout juice liquid medium, and placed in a constant temperature shaker at 37° C. and 180 rpm for 12 hours. Then inoculate the 100mL / 250mL liquid bean sprout juice medium according to 1% of the inoculum, and place it in a constant temperature shaker at 37°C and 180rpm for 24 hours to make a concentration of 2×10 8 cfu / mL bacterial suspension.

[0049] Liquid bean sprout juice medium, its raw material composition is: soybean sprouts 100g, sucrose 50g, water 1000mL, pH natural;

[0050] The preparation method is as follows: boil soybean sprouts in boiling water for 30 minutes, filter with four layers of gauze, and add sucrose to make the volume to 1000 mL.

Example Embodiment

[0051] Example 3: Field test

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Abstract

The invention discloses bacillus atrophaeus antagonistic to lycium chinensis root rot with the classification name of bacillus atrophaeus GQJK17, preserved in CGMCC (China General Microbiological Culture Collection Center) of CCCCM (China Committee of Culture Collection for Microorganisms) on November 10, 2016, with the strain preservation number of CGMCC NO.1 3254. The bacillus atrophaeus GQJK17 obtained through separation has a higher antagonistic effect on the fructus lycii root rot, a new biocontrol bacterium is provided for biocontrol of the lycium chinensis root rot, and the type of biocontrol preparations is increased.

Description

technical field [0001] The invention relates to a strain of bacillus atrophaeus antagonizing wolfberry root rot and application thereof, belonging to the field of biotechnology. Background technique [0002] Lycium barbarum is a famous and precious traditional Chinese medicine. Lycium barbarum fruit has high nutritional, health care and medicinal value. It is also known as "four treasures of traditional Chinese medicine" together with Qiongzhen Ganoderma lucidum, Changbai Mountain ginseng and Dong'e Ejiao. With the development of the economy and the progress of the society, people are more and more aware of the value of wolfberry, prompting people to carry out extensive product development of wolfberry, and achieved important results. Ningxia wolfberry has the largest cultivation area in China. With the continuous expansion of wolfberry planting area, the incidence of wolfberry root rot in old production areas and new production areas has increased year after year, and it ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 丁延芹杜秉海马锦锦姚良同汪城墙刘凯
Owner SHANDONG AGRICULTURAL UNIVERSITY
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