A kind of subculture method of Bulgarian essential oil rose

A technique of subculture and rose, which is applied in the field of subculture and tissue culture of Bulgarian essential oil rose, which can solve the problems of yellowing of tissue culture seedlings, low proliferation ratio, vitrification, etc., so as to increase the ratio of proliferation and reduce the risk of abnormalities , solve the effect of yellowing and growth stagnation of plants

Active Publication Date: 2018-12-25
上海菁艺生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] This experiment aimed at the subculture process of a Bulgarian essential oil rose, and solved the problem of low proliferation ratio, yellowing and vitrification of the tissue culture seedlings in the rapid propagation of a Bulgarian essential oil rose tissue culture, so as to realize the industrialization of Bulgarian essential oil rose tissue culture Production

Method used

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  • A kind of subculture method of Bulgarian essential oil rose
  • A kind of subculture method of Bulgarian essential oil rose

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Experimental program
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Effect test

Embodiment 1

[0035] A method for tissue culture rapid propagation of Bulgarian essential oil rose, comprising the following steps:

[0036] (1) The selection of experimental materials: select the Bulgarian rose tissue culture seedlings with neat growth, plant height of 2.5-4.0cm, subculture cycle of 30-35 days, green or tender green leaves as subculture materials;

[0037] (2) Specific experimental steps:

[0038] (2.1) Acquisition of sterile explants: remove the leaves, keep the terminal buds and heart leaves, leave 0.1-0.2cm of petiole, and cut into 0.5-0.8cm stem segments;

[0039] (2.2) subculture of Bulgarian essential oil rose; terminal buds and stem sections were inoculated separately, and evenly inoculated into subculture medium No. 1 (MG-1);

[0040] Among them, the improved MS (KNO 3 1.9-2.5g / L, NH 4 NO 3 1.65-2.1g / L, MgSO 4 ·7H 2 O0.37-0.48g / L, KH 2 PO 4 0.17-0.22g / L, CaCl 2 2H 2 O 0.44-0.57g / L, Na 2 EDTA·2H 2 O 0.041-0.056g / L, FeSO 4 ·7H 2 O 0.03-0.041g / L, MnSO ...

Embodiment 2

[0047] A method for tissue culture rapid propagation of Bulgarian essential oil rose, comprising the following steps:

[0048] (1) The selection of experimental materials: select the Bulgarian rose tissue culture seedlings with neat growth, plant height of 2.5-4.0cm, subculture cycle of 30-35 days, green or tender green leaves as subculture materials;

[0049] (2) Specific experimental steps:

[0050] (2.1) Acquisition of sterile explants: remove the leaves, keep the terminal buds and heart leaves, leave 0.1-0.2cm of petiole, and cut into 0.5-0.8cm stem segments;

[0051] (2.2) subculture of Bulgarian essential oil rose; terminal buds and stem sections were inoculated separately, and evenly inoculated into subculture medium No. 1 (MG-1);

[0052] Among them, the improved MS (KNO 3 1.9-2.5g / L, NH 4 NO 3 1.65-2.1g / L, MgSO 4 ·7H 2 O0.37-0.48g / L, KH 2 PO 4 0.17-0.22g / L, CaCl 2 2H 2 O 0.44-0.57g / L, Na 2 EDTA·2H 2 O 0.041-0.056g / L, FeSO 4 ·7H 2 O 0.03-0.041g / L, MnSO...

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Abstract

The invention relates to a subculture method of Bulgaria essential oil roses. The method comprises the following steps that (1) experiment materials are selected; (2) concrete experiment steps are implemented: (2.1) sterile explants are obtained through cutting away leaves, reserving terminal bud lobus cardiacus, leaving 0.1 to 0.2 cm of leaf stems, and cutting the leaf stems into stem sections being 0.5 to 0.8cm; (2.3) Bulgaria essential oil rose subculture is performed; the terminal buds and the stem sections are inoculated in a separated way and are uniformly inoculated into a No. 1 subculture medium (MG-1); (2.4) after the culture for 35d, the plant longitudinal growth height is more than 3.5cm; the plant lateral buds all sprout; one lateral bud at the lower part grows to form the single branch with the size being about 2cm; the statistics proliferation proportion averagely reaches 1 to 4.5. The regulation is performed by aiming at the nutrition ingredients required in the rose subculture process; the phenomena of plant yellowing and growth stasis in the rose tissue culture seedling subculture process are avoided; the proliferation proportion of the rose tissue culture seedling is improved. The rose tissue culture system is successfully built; the rose factory production is realized.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a subculture method of Bulgarian essential oil rose, and belongs to the field of biotechnology. Background technique [0002] Bulgarian rose belongs to Rosaceae, Rosa, deciduous shrub. Since the Bulgarian rose is an international fragrance type, the oil yield is as high as 3.6 / 10,000. It is the best variety for extracting rose essential oil and producing rose hydrosol (flower water). The yield of Bulgarian roses is extremely large, reaching 500-750 kg of flowers per mu, and 80-100 kg of dried flowers and buds per mu. [0003] The Bulgarian rose is recognized as a high-quality rose variety in the world. It has a pure and delicate floral fragrance, and belongs to the international light fragrance type. It is widely planted to extract rose oil, which has high economic value. The rose industry in Bulgaria has a history of more than 300 years and is known as the "Kingdom of Roses". The p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 李文剑刘云彦冒文娟张玉杰林大为张承妹苏涌
Owner 上海菁艺生物科技有限公司
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