Method for obtaining baohuoside I by double-phase enzyme hydrolysis

A technology of baohuoside and enzymatic hydrolysis, applied in the field of medicine, can solve the problems of many operation steps, high cost, long cycle, etc., and achieve the effects of reducing side reactions, reducing preparation cost and reducing enzyme dosage.

Active Publication Date: 2017-05-31
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies such as many operation steps, high cost, low yield and long cycle in the existing preparation technology of baohuoside I, and provide a method based on biphasic enzymatic hydrolysis technology to directly obtain baohuoside I from Epimedium. Huoside I method

Method used

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  • Method for obtaining baohuoside I by double-phase enzyme hydrolysis
  • Method for obtaining baohuoside I by double-phase enzyme hydrolysis
  • Method for obtaining baohuoside I by double-phase enzyme hydrolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Weigh 0.1 g of icariin reference substance, add 200 mL of acetic acid-sodium acetate buffer solution (pH 3.5), mix well, and obtain icariin suspension. Add 4.0 g of β-glucosidase to the above icariin suspension and mix evenly, add 100 mL of ethyl acetate, adjust the stirring speed to 200 rpm, heat and react at 50°C for 0.5 h, cool, and divide the ethyl acetate solution, The solvent is recovered under reduced pressure to dryness, and the product of Baohuoside I is obtained. High performance liquid chromatograms before and after enzymatic hydrolysis Figure 4 As shown, it can be seen that icariin has been completely hydrolyzed into baohuoside I under the above conditions, and the yield is 100%.

Embodiment 2

[0036] Weigh 0.1 g of icariin reference substance, add 1000 mL of acetic acid-sodium acetate buffer solution (pH 5.5), mix well to obtain icariin suspension, and add β-glucose to the above icariin suspension Glucosidase 0.5 g, mix well, add 500 mL ethyl acetate, adjust the stirring speed to 50 rpm, heat and react at 40°C for 24 h, cool, separate the ethyl acetate solution, recover the solvent under reduced pressure to dryness, and obtain Baohuoside 1, yield 98.6%.

Embodiment 3

[0038] Weigh 0.1 g of icariin reference substance, add 200 mL of acetic acid-sodium acetate buffer solution (pH 5.0), mix well to obtain icariin suspension, and add β-glucose to the above icariin suspension Glucosidase 2 g, mix well, add 500 mL ethyl acetate, adjust the stirring speed to 150 rpm, heat at 50°C for 2 h, cool, separate the ethyl acetate solution, recover the solvent under reduced pressure to dryness, and obtain Baohuoside I , The yield is 100%.

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Abstract

The invention relates to a method for obtaining baohuoside I by double-phase enzyme hydrolysis and belongs to the technical field of medicines. An extraction process adopted by the method takes icariin as a raw material, and the baohuoside I is obtained by a double-phase solvent system composed of a buffering solution containing an enzyme and ethyl acetate; in the double-phase enzyme hydrolysis method provided by the invention, the enzyme hydrolysis of the icariin and extraction of the baohuoside I are combined to form one step; the icariin is subjected to the enzyme hydrolysis to generate the baohuoside I, and the baohuoside I is transferred to an ethyl acetate layer from a buffering solution layer, so that hydrolysis reaction can be effectively promoted; the method is a method which has high hydrolysis rate and extraction rate and is simple and convenient to operate.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for obtaining baohuoside I by hydrolysis with two-phase enzymes. Background technique [0002] Baohuoside I (Icariside I, also known as Icariside II) is a plant of the genus Epimedium in the family Berberidaceae Epimedium ( Epimedium brevicorum Maxim.), a polyhydroxy flavonoid monomer component, has good anti-tumor and anti-osteoporosis activities, which is obviously superior to other flavonoid glycosides in Epimedium, and has been developed as an anti-inflammatory drug with good curative effect. The future of cancer drugs. [0003] Baohuoside I is the product of removing a molecule of glucose from icariin (see figure 1 ), it was reported that baohuoside I was detected in feces and urine after oral administration of icariin in rats. It is baohuoside I, and it has been reported in literature that its biopharmaceutical properties are significantly better ...

Claims

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Application Information

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IPC IPC(8): C12P19/60
CPCC12P19/60
Inventor 沈玉萍杨欢夏国华周存山王海艳卢艺
Owner JIANGSU UNIV
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