A kit for distinguishing active and latent tuberculosis infection

A technology for active tuberculosis and latent tuberculosis, applied in the field of tuberculosis diagnosis, can solve problems such as failure to achieve diagnostic results, and achieve the effects of high clinical application value, simple testing and low cost

Active Publication Date: 2019-01-29
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The meta-analysis of the existing commercial kits showed that the existing commercial serological detection methods did not achieve the ideal diagnostic effect, whether in the diagnosis of pulmonary tuberculosis or tuberculosis

Method used

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  • A kit for distinguishing active and latent tuberculosis infection
  • A kit for distinguishing active and latent tuberculosis infection
  • A kit for distinguishing active and latent tuberculosis infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Recombinant Rv0189c antigen is used as a detection reagent to distinguish active and latent tuberculosis infection

[0027] 1. Collection of Clinical Specimens

[0028] (1) Collection of medical history data:

[0029] Questionnaire questionnaires were used to investigate the sex, age, BCG vaccination history, previous tuberculosis history, recent close contact history of active tuberculosis, diagnosis of chronic rheumatic diseases, and immunosuppressants 3 months before enrollment. Treatment and other relevant medical history. The sex, age, BCG vaccination history and other basic information of 26 healthy controls who excluded the possibility of new active tuberculosis, chronic rheumatic diseases and HIV infection and had no history of immunosuppressant treatment and previous tuberculosis history were collected. Chest radiographs were taken for all subjects at enrollment to exclude new active tuberculosis, and whether there were old pulmonary tuberculosis l...

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Abstract

The present invention discloses a kit for distinguishing active tuberculosis infection and latent tuberculosis infection. For the kit, an Rv 0189c protein or an analogue of the Rv 0189c protein is used as a diagnostic marker; an ELISA (enzyme-linked immunosorbent assay) method is adopted for testing a blood or body fluid sample of a subject for distinguishing active tuberculosis infection and latent tuberculosis infection; during single use of the Rv 0189c protein, the sensibility is up to 62.4%, and the specificity is 88.4%; and during combined use with other diagnostic antigens (such as 16KD, LAM), the Rv 0189c protein can improve the sensibility of diagnosis. In the kit, only single sample is needed during testing, the assay is easy, fast, free of special laboratory equipment, and low in cost, and a result can be obtained on the same day, so that the kit is suitable for being used for large-scale tuberculosis infection testing in basic level areas.

Description

technical field [0001] The invention belongs to the technical field of tuberculosis diagnosis, and in particular relates to a kit for distinguishing active and latent tuberculosis infection. Background technique [0002] Tuberculosis is one of the major public health problems facing the world today. Its pathogen is Mycobacterium tuberculosis (Mtb), which belongs to the genus Mycobacterium. In recent years, due to the emergence of drug-resistant tuberculosis, human immunodeficiency virus (HIV) combined with Mycobacterium tuberculosis infection, and large-scale population movement, the epidemic situation of tuberculosis has become more serious. At present, about 9 million people worldwide develop active tuberculosis every year, and 2 to 3 million people die of tuberculosis. It is estimated that about 1 / 3 of the world's population is currently infected with Mtb, most of which are latent tuberculosis infection (LTBI), that is, the body's immune system can control the replicatio...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07K14/35G01N33/68
CPCG01N33/6893
Inventor王森吴晶陈嘉臻邵凌云张文宏
OwnerAFFILIATED HUSN HOSPITAL OF FUDAN UNIV