Culture medium for pichia pastoris

Abstract

The invention provides a culture medium for pichia pastoris. The culture medium comprises the following components in parts by weight: 8 parts of tapioca flour, 6 parts of malt flour, 2 parts of celery juice, 1 part of hydrocortisone, 1 part of dried persimmon frost, 1 part of gallnuts and 1 part of chamomile, and on the basis, specific amounts of borneol, yeast powder or peptone can be further added. As certain amounts of traditional Chinese medicine components are added on the basis of common materials of a culture medium, the formula has an outstanding culture effect beyond expectation, is particularly applicable to culture of the pichia pastoris, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for Pichia pastoris. Background technique [0002] Pichia pastoris is a type of methanolotrophic yeast that can use methanol as the only carbon source and energy source. Like other yeasts, it mainly exists in the haploid form during the asexual growth period. When the environment is nutrient-limited, it often induces two conjugative haploid cells with different physiological types to mate and fuse into diploids. Alcohol oxidase required for methanol metabolism in Pichia pastoris is sorted into peroxisomes for compartmentalization When peptone is used as a carbon source, there is only one or a few small peroxisomes in the cell, while when methanol is used as a carbon source, peroxisomes account for almost 80% of the entire cell volume. AOX Increased to 35%-40% of total cell protein. The strain has a certain application in the field of biotechnology, but ...

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the technical defect of prior art, provides a kind of medium that is used for Pichia pastoris, to solve the technical problem that Pichia pastoris proliferates slower in the prior art