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Culture medium for clostridium acetobutylicum

A technology of Clostridium acetobutylicum and culture medium, which is applied in the field of microorganisms, can solve the problems of slow proliferation of Clostridium acetobutylicum, achieve the effect of not easily infecting bacteria and increase the proliferation rate

Inactive Publication Date: 2017-06-13
ZHISHENG TIANJIN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the technical defect of prior art, provides a kind of culture medium that is used for Clostridium acetobutylicum, to solve the technical problem that Clostridium acetobutylicum proliferates slower in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A culture medium for Clostridium acetobutylicum, consisting of the following ingredients in parts by weight: 8 parts of pyruvate, 6 parts of sodium citrate, 2 parts of Chinese yam, 1 part of hydrocortisone, 1 part of stone swallow, and 1 part of borax , 1 part of green tea powder, 1 part of peptone, 1 part of spirulina, 1 part of shikigami. The medium is a liquid medium with a pH of 7.

Embodiment 2

[0017] A culture medium for Clostridium acetobutylicum, consisting of the following ingredients in parts by weight: 8 parts of pyruvate, 6 parts of sodium citrate, 2 parts of Chinese yam, 1 part of hydrocortisone, 1 part of stone swallow, and 1 part of borax , 1 part of green tea powder, 1 part of peptone, 1 part of shikigami. The pH of the medium was 6.

Embodiment 3

[0019] A culture medium for Clostridium acetobutylicum, consisting of the following ingredients in parts by weight: 8 parts of pyruvate, 6 parts of sodium citrate, 2 parts of Chinese yam, 1 part of hydrocortisone, 1 part of stone swallow, and 1 part of borax , 1 part of green tea powder, 1 part of spirulina. The medium is a liquid medium with a pH of 8.

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Abstract

The invention provides a culture medium for Clostridium acetobutylicum, which comprises the following ingredients in parts by weight: 8 parts of pyruvate, 6 parts of sodium citrate, 2 parts of Chinese yam, 1 part of hydrocortisone, stone swallow 1 part, 1 part of borax, 1 part of green tea powder; on this basis, a specific amount of peptone, spirulina or shikigami can be further added. On the basis of the commonly used materials in the culture medium, certain Chinese herbal medicines are added to the formula, which unexpectedly achieves an outstanding culture effect, and is especially suitable for the culture of Clostridium acetobutylicum, with a promising application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for Clostridium acetobutylicum. Background technique [0002] A gram-positive, clostridium-shaped anaerobic bacillus that produces solvents such as acetone and butanol, belonging to the genus Clostridium. Cell size (0.6 ~ 0.9) × (2.4 ~ 4.7) μm, often containing bacterial pyruvate particles. Movement with peripatetic flagella. Spores ovoid, secondary terminal. The colonies on the surface are round and raised, with a diameter of 3-5 mm, irregular edges, off-white color, translucent, and shiny surface. Strictly anaerobic. Can decompose proteins and sugars; use biotin and p-aminobenzoic acid as growth factors. It grows vigorously in corn flour culture solution and can produce a large amount of solvents such as acetone, butanol and ethanol. It is an important industrial fermentation strain. However, Clostridium acetobutylicum has the problem of slow proli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/145
Inventor 季延滨李涛侯继明
Owner ZHISHENG TIANJIN BIOTECH
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