Disposable experimental device and experimental method for inducing carbonate minerals by using sulfate reducing bacteria

Abstract

The invention discloses a disposable experimental device and an experimental method for inducing carbonate minerals by using sulfate reducing bacteria. The experimental device comprises two culture flasks having the same structures, and the walls of the culture flasks are in convex lens shapes having an amplification function; a feed pipe is arranged at the upper part of the wall of each culture flask, and a vent hole and a mineral outlet hole are formed in the bottom of each culture flask; the mouth of each culture flask is provided with an exhaust pipe, a pH electrode inserting hole and an oxidation reduction electrode inserting hole; the culture flasks are respectively and internally provided with lead acetate test paper; the culture flasks, the feed pipe and the exhaust pipe are respectively made from a polystyrene material; the two culture flasks are respectively fixed at the two ends of a stabilizing bracket; one of the two culture flasks is used as an experimental group culture flask, and the other culture flask is taken as a control group culture flask. When being used for culturing anaerobic bacteria in a laboratory by a user, the disposable experimental device is time-saving and labor-saving; furthermore, the disposable experimental device can culture the control group and the experimental group in the same environment, thus avoiding a lot of interference factors.

Description

technical field [0001] The invention relates to the field of biological experimental devices, in particular to a disposable experimental device for sulfate-reducing bacteria inducing carbonate minerals and an experimental method thereof. Background technique [0002] In recent years, due to the rise of geological microbiology, the use of microorganisms to induce minerals has become a research hotspot. Due to the influence of the microorganism itself and its secretions, the minerals induced by microorganisms are significantly different from those obtained by chemical synthesis. In the experiment of sulfate-reducing bacteria-induced carbonate minerals, the methods for cultivating anaerobic bacteria such as sulfate-reducing bacteria are mainly Hungate rolling tube technology, anaerobic glove box technology, etc., due to the requirements of these anaerobic culture technology equipment conditions High, the price is relatively expensive, the operation is relatively cumbersome, an...

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Problems solved by technology

In the experiment of sulfate-reducing bacteria-induced carbonate minerals, the methods for cultivating anaerobic bacteria such as sulfate-reducing bacteria are mainly Hungate rolling tube technology, anaerobic glove box technology, etc., due to the requirements of these anaerobic culture technology equipment conditions High, the price is relatively expensive, and the operation is relatively cumbersome, and the general laboratory does not have the conditions, which limits the extensive development of anaerobic research work

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