A Method for Measuring the Electrical Properties of Cell Suspension Using Electroacoustic Pulse Method
An electro-acoustic pulse method and cell suspension technology, which is applied in the measurement of electricity, electrostatic field measurement, and measurement of electrical variables, etc., can solve the problems of time-consuming, labor-intensive, expensive equipment, and inability to obtain the electrical activity state of cell clusters. Simple equipment and easy operation
Active Publication Date: 2020-01-03
XIJING UNIV
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Problems solved by technology
Although this method has high precision, the equipment is expensive, time-consuming and labor-intensive, and cannot obtain the electrical activity status of the cell cluster as a whole
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Embodiment 1
[0079] Detection of Electrical Properties of Red Blood Cell Suspension in SD Rats
[0080] Experimental materials: A certain amount (50 μL) of fresh blood was taken from the tail of SD rats, sodium heparin was centrifuged for 10 min at 3000 r / min, and the upper serum was discarded. After diluting the erythrocytes with 0.9% normal saline, put them into a centrifuge for 15 minutes at 3000 r / min for the second time, and discard the supernatant. Then the erythrocytes are diluted and prepared into a suspension of erythrocytes with a certain concentration for use.
[0081] Figure 4 It is a micrograph of red blood cells of SD rats, the magnification is 1000. The cell concentration was 6.4 x 10 5 cells / L, basically presenting a single-cell layer arrangement.
[0082] Figure 5 Curves were measured for different volumes of cell suspension samples under an electric field of 10kV / mm. From Figure 5 It can be seen that under the action of a fixed DC bias voltage and a pulsed elect...
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Abstract
The invention relates to a method for measuring the electrical characteristics of a cell suspension by using a pulsed electro-acoustic method. According to the method of the invention, the fundamental principles of a traditional pulsed electro-acoustic method (PEA) for measuring the space charges of a solid thin film or a cable material are adopted to detect the charged characteristics of the cell suspension. The method includes the following steps that: 1) a three-layer composite media model is established, and the use conditions of the three-layer composite media model are defined; 2) red cells are separated from venous blood obtained from the tail of an SD rat, and the red cells are diluted to an appropriate concentration; 3) an obtained red cell suspension is injected into a measuring chamber; 4) direct-current bias voltage and pulse electrical signals are applied to the sample; and 5) acoustic waves are detected and converted into electrical signals through a piezoelectric sensor, so that the charged characteristics of the cell suspension can be calculated. The method of the invention has the advantages of simplicity, quickness and convenient operation. With the method adopted, the charged characteristics of various kinds of cells can be measured in a non-destructive manner, and a new measurement means can be provided for the study of electrophysiology.
Description
technical field [0001] The invention relates to a method for measuring the electrical properties of cell suspension, in particular to a method for measuring the electrical properties of cell suspension by using an electroacoustic pulse method. Background technique [0002] The cell membrane is the pathway for material, energy and information exchange with the environment, and is the most important organelle of the cell. The electrical characteristics of the cell membrane are mainly related to the difference between the ion concentration inside and outside the cell membrane. It is of great significance to study and understand the electrical characteristics of the cell membrane. It is related to the survival of life. development of other disciplines. [0003] At present, the research on the electrical properties of cell membrane mainly includes cell electrophoresis, electrochemical impedance method, patch clamp technique and other methods. Cell electrophoresis method is that...
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IPC IPC(8): G01R31/00G01R29/24G01R29/14
CPCG01R29/14G01R29/24G01R31/00
Inventor马亚红邢卓范小娇黄文准乌伟王娟胡蕊蕊
OwnerXIJING UNIV