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Foot and mouth disease virus and vesicular stomatitis virus identifying duplex fluorescence RT-LAMP (loop-mediated isothermal amplification) detection primer group, kit and application thereof

A technology of RT-LAMP and vesicular stomatitis, which is applied in the field of bovine virus detection, can solve the problems of not being a differential diagnosis, unable to determine the positive reaction result, etc., and achieve the effects of high sensitivity, low interference and good specificity

Inactive Publication Date: 2017-06-27
GUANGXI VETERINARY RES INST
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  • Summary
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Problems solved by technology

At present, the multiple LAMP methods in China have certain limitations, and it is impossible to determine which positive reaction is the result, and it is not a differential diagnosis in the true sense.

Method used

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  • Foot and mouth disease virus and vesicular stomatitis virus identifying duplex fluorescence RT-LAMP (loop-mediated isothermal amplification) detection primer group, kit and application thereof
  • Foot and mouth disease virus and vesicular stomatitis virus identifying duplex fluorescence RT-LAMP (loop-mediated isothermal amplification) detection primer group, kit and application thereof
  • Foot and mouth disease virus and vesicular stomatitis virus identifying duplex fluorescence RT-LAMP (loop-mediated isothermal amplification) detection primer group, kit and application thereof

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Experimental program
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Effect test

Embodiment 1

[0028] 1.1 Materials and methods

[0029] 1.1.1 Virus strains and reagents

[0030] Foot-and-mouth disease inactivated vaccine type A, O type, and Asia 1 type were purchased from Lanzhou Veterinary Research Institute, foot-and-mouth disease inactivated virus type O, A type, Asia 1 type, vesicular stomatitis inactivated virus New Jersey type (NJ type) and Indian type (IND type), bluetongue inactivated virus (type 4, 8, 9, 15, 17, 18), inactivated virus of Peste des petits ruminants (PPRV) was donated by Yunnan Entry and Exit. Three reference strains of bovine viral diarrhea virus (BVDV), two reference strains of bovine rotavirus (BRV), and one reference strain of bovine infectious rhinotracheitis virus (IBRV) were purchased from China Veterinary Drug Control Institute. Enterotoxigenic Escherichia coli (ETEC) and three strains of Mycoplasma bovis (MB) from Guangxi were isolated and preserved in our laboratory. The virus strains and strain sources used in this study are shown in...

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Abstract

The invention belongs to the technical field of bovine virus detection and particularly relates to a foot and mouth disease virus and vesicular stomatitis virus identifying duplex fluorescence RT-LAMP (loop-mediated isothermal amplification) detection primer group, a kit and application thereof. The foot and mouth disease virus and vesicular stomatitis virus identifying duplex fluorescence RT-LAMP detection primer group comprises two groups of specific primers, wherein one group is FMDV-F3, FMDV-B3, FMDV-FIP (F1c-F2) and FMDV-BIP (B1c-B2), and the other group is VSV-F3, VSV-B3, VSV-FIP (F1c-F2) and VSV-BIP (B1c-B2), and sequences of the two groups are shown as SEQ ID NO.1 and SEQ ID NO.8 respectively. An established foot and mouth disease virus and vesicular stomatitis virus identifying duplex fluorescence RT-LAMP method has advantages of simplicity, convenience, quickness, specificity, sensitivity and the like and can be used for FMDV (foot and mouth disease virus) and VSV (vesicular stomatitis virus) clinical detection and epidemiological investigation. The FMDV and VSV duplex fluorescence RT-LAMP method is a simple, quick and low-cost diagnosis method and suitable for large-scale epidemiological investigation.

Description

technical field [0001] The invention belongs to the technical field of bovine virus detection, and in particular relates to a double fluorescent RT-LAMP detection primer set, kit and application thereof for distinguishing foot-and-mouth disease virus and vesicular stomatitis virus. Background technique [0002] Foot-and-mouth disease virus (FMDV) and vesicular stomatitis virus (VSV) are two common highly acute viral infectious diseases of cattle, which are generally explosive and will cause huge losses to the cattle industry once they break out. Economic losses are listed as Class A infectious diseases by the World Organization for Animal Health (OIE). The clinical symptoms caused by FMDV and VSV are very similar, both of which are characterized by salivation, fever, lameness, blisters and ulcers in the mouth, nipples and hooves. The two diseases often have mixed infections and it is difficult to distinguish them. Therefore, it is urgent to establish a rapid differential d...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/6844C12Q1/701C12Q2600/16C12Q2531/119C12Q2537/143C12Q2563/107
Inventor 谢芝勋范晴谢志勤谢丽基黄莉黄娇玲张艳芳曾婷婷王盛罗思思邓显文刘加波庞耀珊
Owner GUANGXI VETERINARY RES INST
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