Foot and mouth disease virus and vesicular stomatitis virus identifying duplex fluorescence RT-LAMP (loop-mediated isothermal amplification) detection primer group, kit and application thereof
A technology of RT-LAMP and vesicular stomatitis, which is applied in the field of bovine virus detection, can solve the problems of not being a differential diagnosis, unable to determine the positive reaction result, etc., and achieve the effects of high sensitivity, low interference and good specificity
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[0028] 1.1 Materials and methods
[0029] 1.1.1 Virus strains and reagents
[0030] Foot-and-mouth disease inactivated vaccine type A, O type, and Asia 1 type were purchased from Lanzhou Veterinary Research Institute, foot-and-mouth disease inactivated virus type O, A type, Asia 1 type, vesicular stomatitis inactivated virus New Jersey type (NJ type) and Indian type (IND type), bluetongue inactivated virus (type 4, 8, 9, 15, 17, 18), inactivated virus of Peste des petits ruminants (PPRV) was donated by Yunnan Entry and Exit. Three reference strains of bovine viral diarrhea virus (BVDV), two reference strains of bovine rotavirus (BRV), and one reference strain of bovine infectious rhinotracheitis virus (IBRV) were purchased from China Veterinary Drug Control Institute. Enterotoxigenic Escherichia coli (ETEC) and three strains of Mycoplasma bovis (MB) from Guangxi were isolated and preserved in our laboratory. The virus strains and strain sources used in this study are shown in...
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