Method for inducing differentiation of hematopoietic stem cells to red blood cells

A technology of hematopoietic stem cells and red blood cells, applied in blood/immune system cells, biochemical equipment and methods, animal cells, etc., can solve the problems of low red blood cell differentiation ratio and complicated operation, and achieve simple and convenient operation, increase differentiation ratio, low cost effect

Inactive Publication Date: 2017-07-21
周婧
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are more complicated to operate, and the proportion of red blood cell differentiation is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Clean the surface of strontium titanate {111} crystal surface single wafer with deionized water in ultrasonic wave; irradiate the cleaned strontium titanate {111} crystal surface single wafer under ultraviolet light (wavelength 185nm, power 40W), The irradiation time was 30min; the ratio of terminal hydroxyl group / bridging hydroxyl group formed in situ on the surface of strontium titanate {111} crystal plane was 4.0; 4 / cm 2 The density of the strontium titanate {111} crystal surface was uniformly planted on a single chip of strontium titanate {111}, and the conventional DMEM basal medium was used for culture, and the medium was changed every two days; after 21 days of culture, mature and differentiated cells were obtained by flow cytometry identification. The proportion of red blood cells was 67%.

Embodiment 2

[0011] Clean the surface of strontium titanate {111} crystal surface single wafer with deionized water in ultrasonic wave; irradiate the cleaned strontium titanate {111} crystal surface single wafer under ultraviolet light (wavelength 185nm, power 40W), The irradiation time was 60min; the ratio of terminal hydroxyl group / bridging hydroxyl group formed in situ on the surface of strontium titanate {111} crystal plane was 4.3; hematopoietic stem cells were divided into 5×10 4 / cm 2 The density of the strontium titanate {111} crystal surface was uniformly planted on a single chip of strontium titanate {111}, and the conventional DMEM basal medium was used for culture, and the medium was changed every two days; after 21 days of culture, mature and differentiated cells were obtained by flow cytometry identification. The proportion of red blood cells was 84%.

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Abstract

The invention discloses a method for inducing differentiation of hematopoietic stem cells to red blood cells. The method comprises irradiating the single crystal surface of strontium titanate crystal {111} to generate a surface with specific hydroxyl properties, and then culturing hematopoietic stem cells on the surface for 21 days through a routine DMEM (Dulbecco's modified eagle medium) to obtain maturely differentiated red blood cells. The method for inducing stem cell directional differentiation through material surface hydroxyl properties is easy and convenient to operate, relatively low in cost and capable of significantly improving the ratio of differentiation of the hematopoietic stem cells to the red blood cells, and meanwhile, can avoid the risks and immunogenicity led by application of animal serums and biochemical reagents, thereby being applicable to the field of treatment on thalassemia, leukemia and the like via stem cell therapy.

Description

technical field [0001] The invention relates to a method for inducing hematopoietic stem cells to differentiate into red blood cells. Background technique [0002] Stem cell directional differentiation regulation is one of the key topics in stem cell research. In addition to biochemical methods such as gene transcription factors, cell growth factors, and chemical inducers, it has been found that the surface properties of materials play a decisive role in mediating the directed differentiation of stem cells in vitro. A new hotspot of research. Hematopoietic stem cells can differentiate into red blood cells, white blood cells, lymphocytes, platelets, and megakaryocytes. Mediating the directed differentiation of hematopoietic stem cells into red blood cells to provide suitable cells for transplantation is an urgent problem to be solved in the fields of cell replacement therapy in the fields of thalassemia and leukemia. Patent applications CN201380080663.2, CN201380080656.2, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078C12N5/0789
CPCC12N5/0641C12N2506/11C12N2537/00
Inventor 周婧
Owner 周婧
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