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Method for inducing differentiation of hematopoietic stem cells towards leukocytes

A technology of hematopoietic stem cells and leukocytes, applied in blood/immune system cells, biochemical equipment and methods, animal cells, etc., can solve the problems of complex operation and low white blood cell differentiation ratio, and achieve simple and convenient operation, increase differentiation ratio, and cost low effect

Inactive Publication Date: 2017-08-11
周婧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are more complicated to operate, and the proportion of white blood cell differentiation is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Clean the surface of the strontium titanate {111} crystal surface single wafer with deionized water in ultrasonic waves; place the cleaned strontium titanate {111} crystal surface single wafer in a muffle furnace for calcination at a temperature of 600°C , the calcination time was 30min; the in situ formation work function on the surface of strontium titanate {111} single crystal surface was 6.0eV; the hematopoietic stem cells were 5×10 4 / cm 2 The density of the strontium titanate {111} crystal surface was uniformly planted on a single wafer of strontium titanate {111}, and the conventional DMEM basal medium was used for culture, and the medium was changed every two days; after 14 days of culture, mature and differentiated cells were obtained by flow cytometry identification. The proportion of white blood cells was 85%.

Embodiment 2

[0011] Clean the surface of the strontium titanate {111} crystal surface single wafer with deionized water in ultrasonic waves; place the cleaned strontium titanate {111} crystal surface single wafer in a muffle furnace for calcination at a temperature of 600°C , the calcination time was 40min; the in situ formation work function on the surface of strontium titanate {111} single crystal surface was 6.3eV; hematopoietic stem cells were 5×10 4 / cm 2 The density of the strontium titanate {111} crystal surface was uniformly planted on a single wafer of strontium titanate {111}, and the conventional DMEM basal medium was used for culture, and the medium was changed every two days; after 14 days of culture, mature and differentiated cells were obtained by flow cytometry identification. The white blood cell ratio was 93%.

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Abstract

The invention discloses a method for inducing differentiation of hematopoietic stem cells towards leukocytes. Strontium titanate {111} crystal-plane single crystal is calcined through heat treatment, so that a surface, which has special work function properties, is generated; then, the hematopoietic stem cells are cultivated on the surface; and the hematopoietic stem cells are cultivated for 14d by virtue of a conventional DMEM basic culture medium, so that leukocytes, which is mature in differentiation, are obtained. The method, which induces directional differentiation of the stem cells on the basis of the work function properties of the material surface, provided by the invention is simple and convenient to operate, quite low in cost and capable of obviously improving a differentiation proportion of the hematopoietic stem cells towards the leukocytes; moreover, the method can avoid risks and immunogenicity caused by application of animal serums and biochemical reagents; and the method is applicable to the fields of treating thalassemia, leukemia and the like withe the adoption of stem cell therapy.

Description

technical field [0001] The invention relates to a method for inducing hematopoietic stem cells to differentiate into white blood cells. Background technique [0002] Stem cell directional differentiation regulation is one of the key topics in stem cell research. In addition to biochemical methods such as gene transcription factors, cell growth factors, and chemical inducers, it has been found that the surface properties of materials play a decisive role in mediating the directed differentiation of stem cells in vitro. A new hotspot of research. Hematopoietic stem cells can differentiate into white blood cells, red blood cells, lymphocytes, platelets, and megakaryocytes. Mediating the directional differentiation of hematopoietic stem cells into leukocytes to provide suitable cells for transplantation is an urgent problem to be solved in the field of cell replacement therapy in the fields of thalassemia and leukemia treatment. Patent applications CN201380080663.2, CN2013800...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0781C12N5/0783C12N5/0786C12N5/0787
CPCC12N5/0635C12N5/0636C12N5/0642C12N5/0645C12N2506/11C12N2533/20C12N2537/00
Inventor 周婧
Owner 周婧
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