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A kind of method that immobilized glucose oxidase prepares sodium gluconate

A technology of glucose oxidase and sodium gluconate, which is applied in fermentation and other directions, can solve the problems of easy inactivation of enzymes, application limitations, and difficulty in repeated use, and achieves the effects of high conversion rate, easy operation and mild conditions.

Active Publication Date: 2020-04-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, enzymes are easily inactivated and difficult to reuse, so their industrial applications, especially in organic synthesis, are limited.

Method used

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  • A kind of method that immobilized glucose oxidase prepares sodium gluconate
  • A kind of method that immobilized glucose oxidase prepares sodium gluconate
  • A kind of method that immobilized glucose oxidase prepares sodium gluconate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Weigh a certain amount of glucose oxidase crude enzyme powder and magnetic cellulose-dimethylimidazolium zinc salt carrier to mix at a mass ratio of 1:10, and immobilize for 1 hour to obtain immobilized glucose oxidase;

[0033] (2) Weighing 18 g of glucose and dissolving it in water to prepare a 100 mM glucose solution, mix it with the immobilized glucose oxidase prepared in step (1), and react at a rotation speed of 200 rpm, pH 7.0, and a temperature of 40° C.;

[0034] (3) reacting for 6 hours until the presence of no glucose can be detected in the reaction solution to end the reaction;

[0035] (4) adding sodium hydroxide to the reaction solution pH is 10 in the reaction solution that step (3) makes;

[0036] (5) Use a magnet to separate the immobilized glucose oxidase in the reaction solution prepared in step (4), wash it and put it into the next use, and rotate the reaction solution, crystallize, and dry to obtain sodium gluconate.

[0037] In this example, t...

Embodiment 2

[0039] (1) Weigh a certain amount of glucose oxidase crude enzyme powder and magnetic cellulose-dimethylimidazolium zinc salt carrier to mix at a mass ratio of 1:10, and immobilize for 1 hour to obtain immobilized glucose oxidase;

[0040] (2) Weigh 36g of glucose and dissolve it in water to prepare a 200mM glucose solution, mix it with the immobilized glucose oxidase prepared in step (1), and react at a rotation speed of 200rpm, pH 7.0, and a temperature of 40°C;

[0041] (3) reacting for 6 hours until the presence of no glucose can be detected in the reaction solution to end the reaction;

[0042] (4) adding sodium hydroxide to the reaction solution pH is 10 in the reaction solution that step (3) makes;

[0043] (5) Use a magnet to separate the immobilized glucose oxidase in the reaction solution prepared in step (4), wash it and put it into the next use, and rotate the reaction solution, crystallize, and dry to obtain sodium gluconate.

[0044] In this example, the convers...

Embodiment 3

[0046] (1) Weigh a certain amount of glucose oxidase crude enzyme powder and magnetic cellulose-dimethylimidazolium zinc salt carrier to mix at a mass ratio of 1:10, and immobilize for 1 hour to obtain immobilized glucose oxidase;

[0047] (2) Weighing 18 g of glucose and dissolving it in water to prepare a 100 mM glucose solution, mix it with the immobilized glucose oxidase prepared in step (1), and react at a rotation speed of 240 rpm, pH 7.0, and a temperature of 40° C.;

[0048] (3) reacting for 6 hours until the presence of no glucose can be detected in the reaction solution to end the reaction;

[0049] (4) adding sodium hydroxide to the reaction solution pH is 10 in the reaction solution that step (3) makes;

[0050] (5) Use a magnet to separate the immobilized glucose oxidase in the reaction solution prepared in step (4), wash it and put it into the next use, and rotate the reaction solution, crystallize, and dry to obtain sodium gluconate.

[0051] In this example, t...

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Abstract

The invention discloses a method for preparing sodium gluconate by immobilizing glucose oxidase, and belongs to the field of the catalyzed reaction application of immobilized enzymes. The method uses a magnetic cellulose-dimethyl imidazole zinc salt as a carrier to immobilize the glucose oxidase, and the immobilized glucose oxidase is used to catalyze the glucose oxidation reaction. The sodium gluconate has the high catalytic efficiency of the immobilized glucose oxidase, and also has the characteristics of high recovery rate of the enzyme activity, simplicity in operation, high stability, and repeated use. The method has the advantages of mild conditions, simplicity in operation, high conversion rate, and realization of repeated use of the catalyst immobilized glucose oxidase through magnet separation. The immobilized glucose oxidase still reserves high enzyme activity after being reacted under optimum conditions at a constant temperature and a constant pH value nine times.

Description

technical field [0001] The invention belongs to the field of application of immobilized enzyme catalytic reaction, in particular to a method for preparing sodium gluconate by immobilized glucose oxidase. Background technique [0002] Sodium gluconate is an extremely versatile polyhydroxy organic acid salt, easily soluble in water. Its molecular formula is C 6 h 11 NaO 7 , the relative molecular mass is 218.14. It is a deep-processing product of glucose. At the same time, it is also the basic raw material for the preparation of gluconate (copper, zinc, ferrous salt) and gluconolactone and other products, and has a wide range of uses in the construction industry, food, water quality stabilizers, and medicine. [0003] Enzymes are important biocatalysts that can convert substrates into products efficiently, specifically and gently. However, enzymes are easily inactivated and difficult to reuse, so their industrial application, especially in organic synthesis, is limited. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/58
CPCC12P7/58
Inventor 宗敏华徐红娄文勇
Owner SOUTH CHINA UNIV OF TECH