Application of glutathione-stabilized gold nano-clusters in detection of cysteine and lysine contents

A technology of glutathione and gold nanoclusters is applied in the field of amino acid detection, which can solve the problems of complicated operation, long reaction time, low sensitivity and selectivity, etc., and achieve the effect of simple preparation method and rapid detection.

Active Publication Date: 2017-08-04
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection of cysteine ​​and lysine is usually affected by long reaction time, reaction conditions such as pH acidity and alkalinity, complex operation, low sensitivity and selectivity

Method used

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  • Application of glutathione-stabilized gold nano-clusters in detection of cysteine and lysine contents
  • Application of glutathione-stabilized gold nano-clusters in detection of cysteine and lysine contents
  • Application of glutathione-stabilized gold nano-clusters in detection of cysteine and lysine contents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation and characterization of glutathione-stabilized gold nanoclusters

[0041] 1. Preparation of gold nanoclusters (GSH-Au NCs) using glutathione as a template

[0042] Using glutathione as a template to synthesize gold nanoclusters with fluorescent properties: the synthesis of fluorescent gold nanoclusters is based on the method reported in the literature [see: Chen W.B., Tu X.J., Guo X.Q.Chem.Commun., 2009, 1736–1738. ], made some improvements, the process is as follows: first, the newly prepared glutathione aqueous solution (5mM, 10mL) and tetrachloroauric acid aqueous solution (5Mm, 10mL) were mixed under vigorous stirring, and then the mixture was reacted for 2~ After 5 days, the product was obtained as a pale yellow solution. In this experiment, the concentration ratio of reactants and raw materials is 1:1, and the reaction conditions are: reaction temperature 30°C, and reaction environment is sunlight or sunlight lighting conditions. After impro...

Embodiment 2

[0047] Example 2 Glutathione-stabilized gold nanocluster fluorescent probe detection of cysteine

[0048] This example will describe in detail how to use the gold nanoclusters of the glutathione template prepared in Example 1 to detect cysteine.

[0049] Use the prepared fluorescent glutathione-stabilized gold nanocluster probe to detect cysteine ​​Specific steps: Take 300 μL of the prepared glutathione-stabilized gold nanocluster sample, add it to a centrifuge tube, and then add a series of concentrations working solution of cysteine, respectively, to detect the effects of different concentrations of cysteine ​​on the fluorescence signal of glutathione-stabilized gold nanocluster probes (final concentrations of 0, 5, 10, 15, 20, 25, 50 , 75, 100, 125, 150, 175, 200, 225, 250, 300 μM). Dilute with double distilled water to a final volume of 3 mL, mix well, and measure. Under the excitation of 402nm wavelength light, the fluorescence spectrum of glutathione-stabilized gold na...

Embodiment 3

[0051] Example 3 Glutathione-stabilized gold nanocluster ratiometric fluorescent probe for detection of lysine

[0052] This example will describe in detail how to use the gold nanoclusters of glutathione template prepared in Example 1 to detect lysine.

[0053] Using the prepared glutathione-stabilized gold nanocluster ratiometric fluorescent probe to detect lysine Specific steps: Take 300 μL of the prepared glutathione-stabilized gold nanocluster sample, add it to a centrifuge tube, and then add a series of concentrations The lysine working solution was used to detect the effects of different concentrations of lysine on the fluorescence signal of glutathione-stabilized gold nanocluster probes (final concentrations were 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5 , 20, 22.5, 25 μM). Dilute with double distilled water to a final volume of 3 mL, mix well, and measure. Under the excitation of 402nm wavelength light, the fluorescence spectrum of glutathione-stabilized gold nanoclusters ...

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Abstract

The invention discloses application of glutathione-stabilized gold nano-clusters in detection of cysteine and lysine contents. The application comprises the following steps: mixing a newly prepared glutathione aqueous solution and a chloroauric acid hydrated aqueous solution under the vigorous stirring in equal concentration and equal volume, and reacting an obtained mixture for 2-5 days at an illumination condition to obtain a light yellow solution, centrifuging, purifying supernate through a dialyser so as to prepare the glutathione-stabilized gold nano-clusters. The glutathione-stabilized gold nano-clusters can be used as a fluorescent probe or a ratiometric fluorescent probe to respectively or simultaneously detect cysteine and lysine. The application of the glutathione-stabilized gold nano-clusters in the detection of the cysteine and the lysine contents has the characteristics of being high in selectivity, high in sensitivity, simple and easy to implement, and the preparation method is simple and convenient, green and gentle.

Description

technical field [0001] The invention belongs to the field of amino acid detection, and in particular relates to the application of glutathione-stabilized gold nanoclusters in detecting the contents of cysteine ​​and lysine. Background technique [0002] Amino acids are the basic constituent units of biological functional macromolecular proteins. The 20 kinds of amino acids in the human body have different functions, promote and influence each other. If the human body lacks any essential amino acid, it can lead to abnormal physiological functions, affect the normal progress of antibody metabolism, and eventually lead to diseases. Diseases caused by amino acid deficiency and metabolic disorders involve various diseases such as immunity, nervous system, cardiovascular and cerebrovascular diseases, kidney, metabolism, diabetes, etc., affecting human growth and development, nutritional health, musculoskeletal growth, hormone secretion, detoxification function and other health ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486G01N2021/6419
Inventor 冯大千刘国良王俊琦张静茹尹倩茹
Owner YANCHENG INST OF TECH
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