Molecular marker method for identifying rice heading date gene qHD7.4

A molecular marker, qhd7.4 technology, applied in the field of biotechnology engineering, can solve problems such as inaccurate identification and achieve the effect of improving adaptability

Inactive Publication Date: 2017-08-08
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES +1
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  • Abstract
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Problems solved by technology

[0008] Aiming at the problem of inaccurate identification caused by the continuous distribution of rice heading stage in the improvement and selection process and being easily affected by environmental factors,

Method used

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  • Molecular marker method for identifying rice heading date gene qHD7.4
  • Molecular marker method for identifying rice heading date gene qHD7.4
  • Molecular marker method for identifying rice heading date gene qHD7.4

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Embodiment Construction

[0031] In order to fully disclose a molecular marker method for identifying the rice heading date gene qHD7.4 of the present invention, the method verification and implementation examples are described below. Its specific implementation steps are as follows:

[0032] (1) Test materials

[0033] All are rice production promotion varieties Guangluai 4 (Guangdong); Nipponbare (Japan); Nanjing 1 (Jiangsu); Nanjing 7 (Jiangsu); Nanjing 14 (Jiangsu); Nanjing 15 (Jiangsu); Nanjing 16 No. (Jiangsu); Huafeng B (Guangdong); II-32B (Hunan); Yuetai B (Guangdong); Sunong 3037 (Jiangsu); Yangdao No. 6 (Guangdong); Minghui 78 (Fujian); IRBB21 ( Philippines); Duohui No. 1 (Sichuan); Yanhui 559 (Jiangsu); Zhenhui 084 (Jiangsu); Shuhui 527 (Sichuan); BG90-2 (Sri Lanka); Gui 630 (Guyana); Minghui 63 (Fujian ); Mianhui 725 (Sichuan); Lehui 188 (Sichuan); Yuejing silk seedlings (Guangdong); Mianhui 501 (Sichuan); Zhehui 7954 (Zhejiang); Guanghui 128 (Guangdong); Fuhui 718 (Sichuan ); Neixianghu...

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Abstract

The invention relates to a molecular marker method for identifying rice heading date gene qHD7.4, and belongs to the technical field of bioengineering. A pair of primers including a forward primer and a reverse primer are designed and synthesized, DNAs of different rice plants are amplified, and if a characteristic band of 117bp can be amplified, the rice plants are homozygote containing a normal heading gene qHD7.4, and if a characteristic band of 109bp can be amplified, the rice plants are homozygote of an early heading gene qhd7.4; if the characteristic band of 117bp and the characteristic band of 109bp exist simultaneously, the rice plants are heterozygote of the qHD7.4 and the qhd7.4. The method provided by the invention can quickly and accurately indentify whether the rice normal heading gene qHD7.4 or the early heading gene qhd7.4 exists in a rice germplasm resource and breeding populations thereof or not, and also can further distinguish the homozygote and heterozygote of qHD7.4, and predict progeny genetype, so as to greatly improve selection efficiency in the rice heading period, and accelerating breeding process.

Description

[0001] 1. Technical field [0002] The invention relates to a molecular marker method for identifying the rice heading stage gene qHD7.4, which belongs to the field of biotechnology engineering and is specially used for the identification of rice germplasm resources containing the qHD7.4 genotype and the selection of new varieties. [0003] 2. Background technology [0004] Rice is the most important food crop in my country, and more than half of the country's population takes rice as a staple food. With the continuous increase of my country's population and the relative decrease of arable land, increasing rice production has become the most important means to ensure my country's food security. Heading date is one of the important agronomic traits of rice. Heading date determines the length of vegetative growth period, affects photosynthetic production and material accumulation, and ultimately directly or indirectly affects rice yield. In recent years, different researchers ha...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 王军周勇杨杰梁国华范方军朱金燕李文奇杨泽峰龚志云王芳权许扬仲维功
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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