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Method for quantitatively determining content of flavonoid components in folium ginkgoes by employing multi-target ingredients

A technology for quantitative determination of flavonoids in leaves, which is applied in measurement devices, material separation, and analysis of materials, etc., can solve the problem that the content of flavonol glycoside prototype components cannot be effectively controlled, and achieves good reproducibility, easy chromatographic conditions, High stability effect

Inactive Publication Date: 2017-08-11
HEILONGJIANG ZBD PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method only tests the three contents of quercetin, kaempferol, and isorhamnetin, and then converts them into the content of total flavonol glycosides, which cannot effectively control the content of flavonol glycosides prototype components
[0005] At present, the research on the quality standards of flavonoids in Ginkgo biloba, Ginkgo biloba extracts and preparations only stays in the use of acid hydrolysis to determine the contents of quercetin, kaempferol and isorhamnetin, and convert them into total flavonol glycosides. In terms of the content method, the control of flavonol glycoside prototype components is blank

Method used

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  • Method for quantitatively determining content of flavonoid components in folium ginkgoes by employing multi-target ingredients
  • Method for quantitatively determining content of flavonoid components in folium ginkgoes by employing multi-target ingredients
  • Method for quantitatively determining content of flavonoid components in folium ginkgoes by employing multi-target ingredients

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Embodiment 1

[0036] Method for Quantitative Determination of Flavonoids Content in Ginkgo Biloba with Multiple Index Components

[0037] 1. Instruments and medicines

[0038] 1.1 Waters 2689 High Performance Liquid Chromatograph

[0039] 1.2 Reagents and medicines

[0040] Acetonitrile is chromatographically pure; phosphoric acid is superior grade; all other reagents are analytically pure; water is ultrapure water.

[0041] 2. Experiment and results

[0042] 2.1 The chromatographic conditions are: the chromatographic column is: Inertsil R ODS-3 C18 250mm×4.6mm 5μm; use octadecylsilane bonded silica gel as filler; use mobile phase A: acetonitrile, mobile phase B: 0.4% phosphoric acid, and carry out gradient elution according to the table below;

[0043]

[0044] The equilibration time is 10 minutes; the flow rate is 1.0m1 per minute; the detection wavelength is 360nm; the column temperature is 48°C;

[0045] 2.2 The method for quantitatively determining the content of flavonoids in...

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Abstract

The invention relates to a method for determining the content of flavonoid components in folium ginkgo extract and particularly discloses a method for quantitatively determining the content of flavonoid components in folium ginkgo extract by employing multi-target ingredients. According to the method, rutin, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, kaempferol-3-O-rhamnose-2-glucoside, quercetin-3-O-rhamnose-2-O-(6-O-p-hydroxy cinnamoyl glucosyl) glucoside and kaempferol-3-O-rhamnose-9-O-6-O-(p-hydroxy cinnamoyl glucosyl) glucoside are taken as reference substances. The method for determining the content of the flavonoid components in the folium ginkgo extract is fast, simple, high in sensitivity, high in accuracy and good in stability, and is capable of accurately and comprehensively controlling the quality of the folium ginkgo extract.

Description

technical field [0001] The invention relates to a method for determining the content of flavonoids in ginkgo leaves, in particular to a method for quantitatively measuring the content of flavonoids in ginkgo leaves with multiple index components. Background technique [0002] Ginkgo biloba is also known as Gongsun tree, ginkgo tree, etc. Its seeds, ginkgo, have a long history in my country as a traditional Chinese medicine for relieving cough, relieving asthma, reducing stool, and stopping turbidity. Its leaves, Ginkgo biloba, also known as ginkgo leaves, are the dried leaves of Ginkgo biloba, a plant of the family Ginkgoceae. The medicinal use of Ginkgo biloba was first recorded in "Shen Nong's Herbal Classic". As early as the Song Dynasty, Chinese folks used Ginkgo biloba to treat asthma and bronchitis. It is recorded in Chinese Pharmacopoeia that Ginkgo biloba has the effects of nourishing the lungs, relieving asthma, and relieving pain, and is used for cough due to lung...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 方同华许照芹项彦华席桂才
Owner HEILONGJIANG ZBD PHARMA
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