Preparation method for microbial agent and application thereof

A technology of microbial inoculum and bacilli, applied in the field of preparation of microbial inoculum, can solve the problems of dead seedlings, microorganisms easily infected with harmful microorganisms, complex microorganism species, etc., and achieve the effects of promoting growth, fast growth and accurate species results.

Inactive Publication Date: 2017-08-18
HUBEI UNIV OF TECH
View PDF5 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The types of microorganisms collected by this method are very complicated, and it is impossible to identify the specific species of microorganisms, and the microorganisms obtained in this way are

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for microbial agent and application thereof
  • Preparation method for microbial agent and application thereof
  • Preparation method for microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] In order to better understand the present invention, the invention will be described in detail below in conjunction with the accompanying drawings and specific examples.

[0032]In order to solve the problems existing in the prior art, the present invention provides a preparation method of microbial bacterial agent, specifically, the dominant bacterial strain is obtained by screening in the original forest soil of Changbai Mountain, and the microbial bacterial strain is made after identifying the species of the dominant bacterial strain The fungicide is applied to the flower plants to promote the growth of the flower plants. The preparation method of a microbial bacterial agent of the present invention and the preferred mode of its application will be described in detail below through specific examples.

[0033] 1) Selection of materials: soil from the virgin forest of Changbai Mountain;

[0034] 2) Dilution coating: take 10 grams of soil, put 10 grams of soil into a c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method for a microbial agent. The method comprises the following steps: 1) taking soil of virgin forest in Changbai Mountain; 2) adopting sterilizing water for diluting the soil for 10 times and then uniformly mixing, adopting a gradient dilution separating method for preparing soil suspension and then respectively coating on five solid medium plates for culturing and selecting one plate with more single colonies as a statistic plate; 3) classifying the colonies on the statistic plate, counting and recording the quantity; 4) confirming three advanced strains according to a result of the step 3); 5) adopting a gram staining method for judging the classification of the colonies of the three advanced strains and then utilizing a PCR technique to expand the species of 16SrDNA identified strains; 6) storing the advanced strains; 7) culturing the three advanced strains, mixing at proper volume ratio and then centrifuging and suspending in the sterilized saline, thereby acquiring the microbial agent. The prepared microbial agent can be used for promoting the growth of flowers and plants. The method is simple, the operation is easy and the prepared microbial agent can obviously promote the plant growth.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a preparation method and application of a microbial bacterial agent. Background technique [0002] Fertilizers for flower cultivation are the source of nutrients for flowers. Whether the fertilization is reasonable or not directly affects the production of flowers, which is related to the yield and quality of flowers. Plants need more elements for growth and development, the main components are nitrogen, phosphorus, potassium, nitrogen, phosphorus, and potassium are not enough to be supplied by cultivation soil alone, so they need to be supplemented by fertilization. [0003] Existing fertilizers are divided into organic fertilizers, inorganic fertilizers and microbial fertilizers. Organic fertilizers are dirty and smelly, which is unacceptable to flower farmers. Although inorganic fertilizers are clean and hygienic, long-term use will easily cause soil compaction. Microbial...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/02A01N63/00A01P21/00C12R1/01C12R1/38
CPCC12N1/20A01N63/00C12N1/02
Inventor 宫春杰胡征张华山
Owner HUBEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap