Preparation method for microbial agent and application thereof

Abstract

The invention discloses a preparation method for a microbial agent. The method comprises the following steps: 1) taking soil of virgin forest in Changbai Mountain; 2) adopting sterilizing water for diluting the soil for 10 times and then uniformly mixing, adopting a gradient dilution separating method for preparing soil suspension and then respectively coating on five solid medium plates for culturing and selecting one plate with more single colonies as a statistic plate; 3) classifying the colonies on the statistic plate, counting and recording the quantity; 4) confirming three advanced strains according to a result of the step 3); 5) adopting a gram staining method for judging the classification of the colonies of the three advanced strains and then utilizing a PCR technique to expand the species of 16SrDNA identified strains; 6) storing the advanced strains; 7) culturing the three advanced strains, mixing at proper volume ratio and then centrifuging and suspending in the sterilized saline, thereby acquiring the microbial agent. The prepared microbial agent can be used for promoting the growth of flowers and plants. The method is simple, the operation is easy and the prepared microbial agent can obviously promote the plant growth.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a preparation method and application of a microbial bacterial agent. Background technique [0002] Fertilizers for flower cultivation are the source of nutrients for flowers. Whether the fertilization is reasonable or not directly affects the production of flowers, which is related to the yield and quality of flowers. Plants need more elements for growth and development, the main components are nitrogen, phosphorus, potassium, nitrogen, phosphorus, and potassium are not enough to be supplied by cultivation soil alone, so they need to be supplemented by fertilization. [0003] Existing fertilizers are divided into organic fertilizers, inorganic fertilizers and microbial fertilizers. Organic fertilizers are dirty and smelly, which is unacceptable to flower farmers. Although inorganic fertilizers are clean and hygienic, long-term use will easily cause soil compaction. Microbial...

AI Technical Summary

Problems solved by technology

The types of microorganisms collected by this method are very complicated, and it is impossible to identify the specific species of microorganisms, and the microorganisms obtained in this way are very easy to infect harmful microorganisms. Once this bacterial agent infected with harmful microorganisms is used on flowers or When in crops, it can cause serious consequences of dead seedlings

Images