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Main-effect QTL molecular marker of corn No.4 chromosome head row number, method for assistant selection of multi-row corn and application of main-effect QTL molecular marker

A technology of molecular markers and number of spike lines, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of different research results, achieve high selection efficiency and clear selection goals , The effect of simple identification method

Active Publication Date: 2017-08-18
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

Although a lot of research has been done on the QTL of panicle row number at home and abroad, the research results are not the same due to the differences in experimental materials, genetic populations, linkage maps, and QTL analysis methods.

Method used

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  • Main-effect QTL molecular marker of corn No.4 chromosome head row number, method for assistant selection of multi-row corn and application of main-effect QTL molecular marker

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Experimental program
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Embodiment Construction

[0011] The experimental methods in the following examples are conventional methods unless otherwise specified, and the involved experimental reagents and materials are conventional biochemical reagents and materials unless otherwise specified.

[0012] 1. The detailed steps for obtaining the main effect QTL molecular markers for controlling the number of corn ear rows are as follows:

[0013] (1) Construction of maize triple testcross (TTC) population and identification of ear row number

[0014] TC(B73), TC(Mo17) and TC(F1)TTC populations were obtained by crossing 121 IBM recombinant inbred line populations with high genetic exchange rates of different genotypes with their parents B73, Mo17 and F1 respectively. 121 different genotype IBM populations, TC(B73), TC(Mo17) and TC(F1) populations were planted in the experimental base according to random grouping. Each genotype material was planted in a single row with a row length of 3.6 m, a row spacing of 60 cm, and a plant spac...

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Abstract

The invention belongs to the technical field of molecular breeding, and particularly relates to a main-effect QTL molecular marker of corn No.4 chromosome head row number, a method for assistant selection of multi-row corn and application of the main-effect QTL molecular marker. The main-effect QTL molecular marker for controlling corn head row is composed of an InDel 73 primer and an umc1667 primer, and used for breeding multiple head rows of corns. Through the molecular marker disclosed in the invention, the assistant selection of the multi-row corn can be carried out; the head row number can be predicted only by detecting a characteristic amplification strip of the molecular marker; the identifying method is simple and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding, and in particular relates to a main-effect QTL molecular marker for the number of ear rows on chromosome 4 of corn, a method for assisting selection of corn with multiple ear rows and an application thereof. Background technique [0002] Maize is one of the major field crops in the world. Corn grains can be used as food and feed, and straw can be used as energy materials, which play an important role in ensuring food security and developing energy. In recent years, the planting area of ​​corn in China has been increasing year by year. As of 2014, the national corn planting area has reached 37 million hectares. Although the country has carried out agricultural supply-side reforms, optimized the crop planting structure, and reduced the planting area of ​​non-maize main producing areas, the total corn planting area after the reduction is still huge. In order to ensure the total amount ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张体付赵涵
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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