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A kind of electrochemiluminescence biochemical detection system and method based on mobile phone usb-otg interface

A USB-OTG, biochemical detection technology, applied in the field of electrochemiluminescence biochemical detection systems, can solve the problems that can only occur between the master device and the slave device, but cannot occur between the slave device and the slave device, etc. Concentration-dependent, good conductivity, portable and versatile effects

Active Publication Date: 2019-05-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the standard USB adopts a master-slave structure, that is, a master device is connected to one or more slave devices. In a transmission system, data transmission can only occur between the master device and the slave device, not between the slave device and the slave device. Between devices

Method used

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  • A kind of electrochemiluminescence biochemical detection system and method based on mobile phone usb-otg interface
  • A kind of electrochemiluminescence biochemical detection system and method based on mobile phone usb-otg interface
  • A kind of electrochemiluminescence biochemical detection system and method based on mobile phone usb-otg interface

Examples

Experimental program
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Effect test

Embodiment 1

[0075] Embodiment 1: BSA electrochemiluminescence detection based on mobile phone, specifically comprises the following steps:

[0076] (A) Anti-BSA modification of ITO electrode: the clipping area is 1cm 2 Dissolve nitrocellulose film in 1mL of methanol, stir until the nitrocellulose film is completely dissolved; take 10uL of dissolved nitrocellulose film and drop it on the conductive surface of ITO conductive glass, and spread it evenly in the middle to form an area of ​​3cm ×3cm square modification area, let it stand for 1 minute until the methanol volatilizes, and form a sensitive area modified by nitrocellulose film in the middle area of ​​ITO; drop 200uL of 1mM anti-BSA in this area, and evenly spread it into an area of ​​3cm ×3cm square modification area, let it stand for 30 minutes, use the adsorption of nitrocellulose film, modify the anti-BSA on the surface of ITO electrode, in the subsequent electrochemiluminescence reaction, it can specifically react with BSA to ca...

Embodiment 2

[0080] Embodiment 2: Electrochemiluminescent detection of thrombin based on mobile phone, specifically comprising the following steps:

[0081] (A) The thrombin-specific response peptide modification of the ITO electrode: the clipping area is 1cm 2 Dissolve nitrocellulose film in 1mL of methanol, stir until the nitrocellulose film is completely dissolved; take 10uL of dissolved nitrocellulose film and drop it on the conductive surface of ITO conductive glass, and spread it evenly in the middle to form an area of ​​3cm ×3cm square modified area, let it stand for 1 minute until the methanol volatilizes, and form a sensitive area modified by nitrocellulose film in the middle area of ​​ITO; drop 200uL of 10 -5 M's thrombin-specific response polypeptide (Cys-Leu-Val-Pro-Arg-Gly-Ser-Cys), and spread evenly into a square modified area with an area of ​​3cm×3cm, let it stand for 30 minutes, and use a nitrocellulose film The adsorption effect of thrombin on the ITO electrode surface m...

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Abstract

The invention discloses an electrochemical luminescence biochemical detection system based on a USB-OTG interface of a mobile phone and a method. An electrochemical luminescent adjustable excitation source system based on the USB-OTG interface of the mobile phone is designed for generating electrochemical luminescence; an electrochemical luminescent analyzing unit is designed for quantitatively analyzing the electrochemical luminescence; the influence of different voltages on the electrochemical luminescence and the concentration selection of terpyridyl in an electrochemical luminescence process are researched, so that the optimization value of the voltage and terpyridyl ruthenium concentration can be acquired and can be used for subsequent electrochemical luminescent detection based on the mobile phone; and finally, the electrochemical luminescence analysis for BSA and thrombin is realized on the mobile phone through different electrode modifications. The invention realizes a stimulating and analyzing integrated mobile phone electrochemical luminescence analysis platform, and the technology is applied to the electrochemical luminescence detection process for BSA and thrombin, so that the electrochemical luminescence biochemical detection system has the advantages of portable general device, high stability, convenience in operation, and the like and can meet the requirement for instant biochemical detection.

Description

technical field [0001] The invention relates to an electrochemiluminescence biochemical detection system and method based on a USB-OTG interface of a mobile phone, and in particular realizes electrochemiluminescence analysis integrating voltage excitation and image analysis on a mobile phone platform. Background technique [0002] USB is hot-swappable and easy to carry and use. At the same time, the USB standard is also an open standard. It supports different devices from different manufacturers to be used on the same device. It has a wider range of application scenarios and supports different devices. Based on the above characteristics, the USB interface has been rapidly popularized. However, the standard USB adopts a master-slave structure, that is, a master device is connected to one or more slave devices. In a transmission system, data transmission can only occur between the master device and the slave device, not between the slave device and the slave device. between d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/48
CPCG01N21/76G01N27/48
Inventor 刘清君李爽刘磊
Owner ZHEJIANG UNIV
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