Allopolyploid induction production method of hybridized Chinese pennisetum

A technique of hybridizing pennisetum and polyploidy, applied in gardening methods, botanical equipment and methods, plant regeneration, etc., to achieve the effect of thick stems, high stem color, and short internodes

Inactive Publication Date: 2017-09-15
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for hybrid pennisetum, there is no repo

Method used

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  • Allopolyploid induction production method of hybridized Chinese pennisetum
  • Allopolyploid induction production method of hybridized Chinese pennisetum
  • Allopolyploid induction production method of hybridized Chinese pennisetum

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Embodiment 1

[0036] The polyploid induction production of hybrid pennisetum was carried out by the following method:

[0037] 1. Culture medium preparation and sterilization

[0038] Primary medium:

[0039] ①N6+6-BA3.0mg / L+chlormequat 3.0mg / L+3% sucrose+0.75% agar;

[0040] ②N6+6-BA4.0mg / L+chlormequat 5.0mg / L+3% sucrose+0.75% agar;

[0041] ③N6+6-BA5.0mg / L+chlormequat 5.0mg / L+3% sucrose+0.75% agar.

[0042] Proliferation medium:

[0043] ①1 / 2MS+6-BA1.0mg / L+paclobutrazol 5.0mg / L+3% sucrose+0.75% agar;

[0044] ②1 / 2MS+6-BA2.0mg / L+paclobutrazol 5.0mg / L+3% sucrose+0.75% agar;

[0045] ③1 / 2MS+6-BA2.5mg / L+paclobutrazol 3.0mg / L+3% sucrose+0.75% agar.

[0046] The above medium was sterilized at 121°C for 20 minutes.

[0047] Rooting medium:

[0048] ①1 / 2MS+paclobutrazol 0.5mg / L+2% sucrose+0.75% agar;

[0049] ②1 / 2MS+paclobutrazol 1.0mg / L+2% sucrose+0.75% agar;

[0050] ③1 / 2MS+paclobutrazol 1.5mg / L+2% sucrose+0.75% agar;

[0051] ④1 / 2MS+paclobutrazol 2.0mg / L+2% sucrose+0.75% agar;

[005...

experiment Embodiment 1

[0072] In this experimental example, three methods of mutagen solution immersion, medium addition method, solution immersion and medium addition method were used to conduct polyploid induction experiments. Each method set several sets of treatment time and concentration The effects of different temperatures, different treatment methods, different concentrations of mutagens and different treatment times on the polyploid induction rate were studied.

[0073] A, mutagen solution immersion method: get hybrid pennisetum (Juncus effususL.) allotriploid hybrid first generation seed, after being sterilized on the aseptic operating table, soak in colchicine ( 1.0mg / L, 2.0mg / L, 3.0mg / L) and dimethyl sulfoxide (1.5mg / L, 2.0mg / L) mutagen soaked for 30min, 60min, 90min, the treatment temperature was 4°C, Then take it out and clean it up, and inoculate it on the primary culture medium ②, the number of inoculated explants in each treatment is 10, 30 bottles, each repeated three times.

[00...

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Abstract

The invention relates to an allopolyploid induction growth method of hybridized Chinese pennisetum. The method comprises the following steps: immersion of allotriploid hybridized Chinese pennisetum F1 seeds in a mutagen containing colchicine and dimethylsulfoxide, initial culture, polyploid identification, proliferation culture, expanded production and transplanting. The method allows a large number of mutagenic seedlings to be obtained in a short time, greatly improves the culture efficiency and saves the production cost.

Description

technical field [0001] The invention relates to the technical field of plant breeding and tissue culture, in particular to an allopolyploid induction production method of hybrid pennisetum. Background technique [0002] Hybrid Pennisetum americanum×P.purpureum belongs to the perennial forage grass of the grass family Pennisetum genus, which is produced by tetraploid elephant grass (Pennisetum purpureum Schum) and diploid American pennisetum (Pennisetumalopecuroides (L.) Spreng) Interspecific allotriploid hybrids are usually produced by vegetative propagation or hybrid generation seeds. Hybrid pennisetum combines the excellent traits of both parents and has obvious heterosis. The content of crude fat, crude protein, crude fiber, nitrogen-free extract and ash in the fresh grass is high, and it is rich in nutrition. It is a high-quality and high-yield suitable for feeding cattle High-quality green feed for various herbivores such as sheep, fish, etc. In addition, hybrid penni...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001A01H4/008
Inventor 黄丽芳孙鏖夏新界邓荟芬易康乐
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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