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Method for injecting bile acid into embryo eggs for improving growth of hatched chicks

A technology for bile acid and breeding eggs, which is applied in the field of poultry breeding, achieves the effects of improving the efficiency of chicken breeding, increasing market value, and improving the prospect of popularization and application.

Inactive Publication Date: 2017-09-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are no reports on the effects of bile acids on the growth and development of poultry embryos

Method used

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  • Method for injecting bile acid into embryo eggs for improving growth of hatched chicks
  • Method for injecting bile acid into embryo eggs for improving growth of hatched chicks
  • Method for injecting bile acid into embryo eggs for improving growth of hatched chicks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The effect of embodiment 1 embryonic egg injection CDCA on chicken body weight, leg muscle rate and chest muscle rate after hatching

[0043] 1. In order to study the direct effect of embryo-egg injection of bile acid on the growth and muscle development of offspring chickens, we designed an experiment of embryo-egg injection.

[0044] Specifically, the method for embryo egg injection comprises the following steps:

[0045] (1) Select 240 purebred recessive whitelock eggs (provided by Wen's Breeder Farm) with an egg weight in the range of 55±5g, and perform conventional incubation under the same conditions.

[0046] Before hatching, the eggs were randomly divided into four groups, which were PBS group, DMSO group, low-dose CDCA group and high-dose CDCA group, and were weighed, sterilized and numbered.

[0047] (2) The breeding eggs collected in (1) were hatched in the Laboratory of Animal Molecular Nutrition, School of Animal Science, South China Agricultural Universit...

Embodiment 2

[0075] Example 2 Effect of CDCA injection on embryonic egg on the development of leg muscle fiber and the expression of myogenic factors mRNA

[0076] 1. In order to study the effect of CDCA injection on leg muscle tissue muscle fiber distribution and myogenic factors, the H&E staining test of muscle fiber sections was carried out.

[0077] 2. The result is as follows image 3 and Figure 4 : The test results showed that low-dose CDCA injected into embryo eggs significantly increased the number of leg muscle fibers per unit area at E16 and DOH, and high-dose CDCA significantly increased the diameter of broiler muscle fibers at D42. Egg injection of CDCA can up-regulate the expression of decorin, and then promote the development of skeletal muscle fibers by regulating the expression of myogenic factors, and improve the meat production ability of broilers.

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Abstract

The invention discloses a method for injecting bile acid into embryo eggs for improving growth of hatched chicks. The first study discovers that the bile acid which is directly injected into the embryo eggs can obviously promote muscle development of chick embryos and growth of the hatched chicks, and is non-toxic and harmless to growth of poultry, and the market value of the poultry can be effectively improved. Moreover, with the adoption of a manner of directly injecting the bile acid into the embryo eggs, the method disclosed by the invention is convenient and rapid in process, does not have any special equipment requirement, provides a novel method capable of increasing yield and increasing culture benefits for the chicken breeding industry, and has excellent popularization and application prospects.

Description

technical field [0001] The invention belongs to the technical field of poultry breeding. More specifically, it relates to a method for injecting bile acid into the embryo to promote the growth of hatched chicks. Background technique [0002] Physiological regulators are substances that are artificially given to animals with different uses for some special needs, and their functions are to promote the growth and development of poultry, prevent diseases, improve the utilization rate of feed and the quality of meat products, etc. Poultry can produce nutritious meat products at a lower cost in a short production cycle, and is the main source of animal protein food for humans. Therefore, improving the muscle growth potential of poultry through the use of physiological regulators has always been an important goal pursued by the poultry industry. [0003] The composition of total bile acids includes chenodeoxycholic acid (CDCA), lithocholic acid (LCA), deoxycholic acid (DCA) and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K45/00
CPCA01K45/007
Inventor 王丽娜江青艳曾庆节左旭冬束刚朱晓彤王松波
Owner SOUTH CHINA AGRI UNIV
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