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A kind of antibody incubation kit and using method thereof

A kit and antibody technology, applied in the field of laboratory equipment, can solve the problems of difficult to deal with multiple antibody incubation, incomplete antibody recovery, and difficulty in removing air bubbles, and achieve the effect of convenient recovery of antibodies, complete recovery of antibodies, and prevention of evaporation.

Active Publication Date: 2019-06-11
XIAMEN LIFEINT TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In Western blotting, the membrane and antibody are generally placed in a small box and incubated on a shaker. This method requires a large amount of antibody each time; it is difficult to deal with multi-antibody incubation; the recovery of antibodies is not complete. Depending on the material of the small box, recovery have different efficiencies
At present, another antibody incubation method is to place the membrane and antibody in a small bag made of plastic film. This method causes a lot of waste of time and antibody when adding samples and antibody recovery, and it is difficult to remove it, which has a great impact on the experiment. Bubbles; In addition, the plastic film is used one-time, resulting in waste of resources and environmental pollution

Method used

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  • A kind of antibody incubation kit and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 antibody kit

[0029] 1. Take a sealable box and lay a 5mm thick silicone rubber 2 inside to form a horizontal plane in the box body 1.

[0030] 2. Cut out the impermeable film 4 whose length and width are slightly larger than the PVDF membrane 6, arrange the impermeable film 4 on a suitable position on the plane of the silicone rubber 2 and lay it flat. Using the plasticity of polymethylvinyl siloxane, apply pressure to the middle of the 4 surfaces of the impermeable film, so that the bottom surface sinks by 2-3mm. So far, the bottom of the water-impermeable film 4 is flat to form a groove 5, and the groove 5 and the water-impermeable film 4 are closely attached.

[0031] 3. Wrap a wet tissue 3 around the inner wall of the airtight box to prevent the antibody diluent from evaporating.

[0032] In the embodiment, the water-impermeable film can be a sealing film, a plastic wrap or tinfoil.

[0033] The silicone rubber in the embodiment ...

Embodiment 2

[0034] The use of embodiment 2 antibody kit

[0035] Take western blotting as an example:

[0036] After the hybridization membrane 6 was transferred to the membrane and rinsed, the PVDF membrane with the protein-containing side facing up was laid horizontally in the groove 5 of the kit of Example 1, and the antibody diluent was directly added dropwise. The amount of antibody diluent added is 3-4mL antibody diluent / (6cm×8cm) hybridization membrane. The volume of antibody diluent should not be larger than groove 5. The hybridization membrane 6 is soaked in the antibody diluent to carry out the hybridization reaction. The incubation process can be kept horizontally or shaken slowly at a small angle.

[0037] Antibody recovery: After lifting a corner to take away the hybridization membrane, the antibody solution remains in the groove 5 formed by the impermeable membrane 4, and can be directly sucked up quickly with a pipette gun.

[0038] In the embodiment, the hybrid membran...

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Abstract

The present invention discloses an antibody incubation kit and a use method thereof. The antibody incubation kit comprises a box body and a box cover, wherein the box body comprises a silicone rubber, water-impermeable films, a wet wipe and concave grooves, the silicone rubber is arranged in the box body, the water-impermeable film is paved on the silicone rubber, the water-impermeable film is provided with the concave groove, and the inner wall of the box body is covered with the wet wipe. The use method comprises: arranging the concave groove having the height of less than the plane of the silicone rubber on the water-impermeable film paved on the silicone rubber, carrying out film transferring on a hybrid film, rinsing, horizontally paving the hybrid film in the concave groove by making the hybrid film surface containing protein upward, adding an antibody dilution liquid in a dropwise manner to immerse the hybrid film into the antibody dilution liquid, and carrying out a hybridization reaction. According to the present invention, the use method has characteristics of simple operation, good effect, and low cost.

Description

technical field [0001] The invention belongs to the field of experimental equipment, and in particular relates to an antibody incubation kit and a use method thereof. Background technique [0002] Western blotting, southern blotting and other blot hybridization methods are often used in biological experiments to detect target bands. In the incubation process of hybridization, the traditional method is to completely immerse the membrane where the molecule is located in the liquid containing the antibody, and make the antibody bind to the target molecule on the membrane after >1 hour. [0003] In Western blotting, the membrane and antibody are generally placed in a small box and incubated on a shaker. This method requires a large amount of antibody each time; it is difficult to deal with multiple antibody incubations; the recovery of antibodies is not complete. Depending on the material of the small box, recovery efficiencies vary. At present, another antibody incubation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 陈欣欣陈茂立许剑雄叶国栋陈巍魏李彬肖剑萍徐晓政付伟
Owner XIAMEN LIFEINT TECH CO LTD
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