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Lateral flow testing sheet and detection method thereof

A test piece and lateral flow technology, applied in the field of detection, can solve the problems of low concentration, low sensitivity and difficult detection of biomarkers

Active Publication Date: 2017-11-21
上海艾瑞德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the sensitivity of related products on the market is low, and the concentration of most biomarkers is low, making it difficult to detect by conventional detection methods

Method used

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  • Lateral flow testing sheet and detection method thereof
  • Lateral flow testing sheet and detection method thereof
  • Lateral flow testing sheet and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0132] Embodiment 1: Determination of CRP (C-reactive protein) in blood

[0133] The sample to be tested is dropped onto the sample pad of the test piece, so that the sample to be tested flows toward the absorbent pad and flows through the binding area, the first quality control area, the detection area and the second Second quality control area.

[0134] The sample flows from the sample pad to the binding area (containing the colloidal gold substance of the labeled mouse anti-human CRP monoclonal antibody), and the antibody is dialyzed, reaches the chromatographic membrane, and capillary phenomenon occurs on the absorbent pad.

[0135] If there is CRP in the sample, it will form a complex with the labeled anti-human CRP colloidal gold substance in the binding area, and then react with the labeled anti-human CRP colloidal gold substance on the detection line T (test area), resulting in a red line.

[0136] At the same time, no matter whether there is CRP or not, the labeled r...

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Abstract

The present invention provides a lateral flow testing sheet and a detection method thereof. The lateral flow testing sheet comprises a sample pad, a bonding zone, a first quality control zone, a detection zone, a second quality control zone and an absorption pad, wherein the first quality control zone is a narrowing zone and has a radial width d1, the detection zone is a narrow zone and has a radial width d2, the second quality control zone is an enlargement zone and has a radial width d3, the radial width of the binding zone is d0, the radial width of the absorption pad is d4, the mean value of d4 is more than the mean value of d3, the mean value of d3 is more than the mean value of d2, the mean value of d0 is more than the mean value of d1, and the mean value of d1 is more than the mean value of d2.

Description

technical field [0001] The invention belongs to the detection field, in particular, the invention relates to a lateral flow test piece, a detection method and an application thereof. Background technique [0002] Immunochromatography takes human or animal body fluid components as the measurement object, detects biomarkers in body fluids, and reads and quantifies the reaction lines or the shades of spots. The reflection photometry method of the test paper is applied to the determination of urine and blood samples. At present, such test strips, measuring instruments and daily urine test strips are widely used in residents' health diagnosis and hospital screening. Check items such as sugar, protein, bilirubin, hemoglobin, urobilin, etc. A detection line and a quality control line are set on the test piece, wherein the detection line is used to quantitatively indicate the concentration of the analyte, and the control line is used for quality control. At present, the sensitivi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/52
CPCG01N33/52G01N33/558
Inventor 李欢黄国栋廖玲妮宁晨曦王陈成
Owner 上海艾瑞德生物科技有限公司
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