Lateral flow testing sheet and detection method thereof
A test piece and lateral flow technology, applied in the field of detection, can solve the problems of low concentration, low sensitivity and difficult detection of biomarkers
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[0132] Embodiment 1: Determination of CRP (C-reactive protein) in blood
[0133] The sample to be tested is dropped onto the sample pad of the test piece, so that the sample to be tested flows toward the absorbent pad and flows through the binding area, the first quality control area, the detection area and the second Second quality control area.
[0134] The sample flows from the sample pad to the binding area (containing the colloidal gold substance of the labeled mouse anti-human CRP monoclonal antibody), and the antibody is dialyzed, reaches the chromatographic membrane, and capillary phenomenon occurs on the absorbent pad.
[0135] If there is CRP in the sample, it will form a complex with the labeled anti-human CRP colloidal gold substance in the binding area, and then react with the labeled anti-human CRP colloidal gold substance on the detection line T (test area), resulting in a red line.
[0136] At the same time, no matter whether there is CRP or not, the labeled r...
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