Method for detecting nitrofuran veterinary drug residues in gelatin

A detection method and a technology for veterinary drug residues are applied in the detection field of nitrofuran veterinary drug residues, which can solve the problems of poor reproducibility of measurement results, low activity of small molecules, and difficulty in detection, and achieve full derivatization of samples, improved sensitivity, Practical effect

Inactive Publication Date: 2017-12-12
广东省药品检验所
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Problems solved by technology

The parent drug of nitrofurans is difficult to detect due to instability; the small molecules produced by the metabolism of the parent drug are also difficult to detect due to low activity; the European Union requires that metabolites be used as target analytes for the detection of nitrofuran drug residues, and the sensitivity of the method must be high. reached 1 μg/kg, and Chi

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  • Method for detecting nitrofuran veterinary drug residues in gelatin
  • Method for detecting nitrofuran veterinary drug residues in gelatin
  • Method for detecting nitrofuran veterinary drug residues in gelatin

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Embodiment 1

[0044] Example 1 A detection method for nitrofuran veterinary drug residues in gelatin

[0045] (1) Preparation of internal standard solution: take AOZ-D 4 、AMOZ-D 5 , AHD- 13 C 3 , SEM. HCl( 13 C, 15 N 2 ) appropriate amount of reference substance, and use methanol to make four internal standard mixed solutions containing 20ng per 1mL.

[0046] (2) Preparation of the test solution:

[0047] Take 2.0g of gelatin (or intermediate) sample, add 1.0g of trypsin, add 100μl of 20ng / ml internal standard solution, then add 20ml of 0.2mol / L hydrochloric acid solution, and finally add 100mmol / L 2-NBA (o-nitrobenzaldehyde ) 100 μl, mixed, shaken for 5-10 min, and kept in a constant temperature water bath at 37° C. overnight (14-16 h).

[0048]Cool the above sample to room temperature, add 1ml of 0.3mol / L sodium phosphate solution, adjust the pH to 7.4±0.2 with 2mol / L NaOH solution, add 5ml of ethyl acetate, vortex for 10-20min, centrifuge at 4000r / min for 5min, take For the eth...

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Abstract

The invention discloses a method for detecting nitrofuran veterinary drug residues in gelatin. Through the method of adding enzymes, sample pretreatment is improved, and the method is suitable for the detection of nitrofuran residues in gelatin and intermediate samples in the production process of gelatin. According to the method for detecting the nitrofuran veterinary drug residues in gelatin, through the method of adding the enzymes, sample pretreatment is improved, the derivatization is more adequate and uniform, the measurement recovery rate and the accuracy are improved, the operation process is simpler and quicker, the sensitivity of the detection method can be significantly improved, the practicality is high, and support is provided for the prevention and control of the nitrofuran veterinary drug residues in gelatin and the production process of gelatin.

Description

technical field [0001] The invention relates to a method for detecting nitrofuran veterinary drug residues in gelatin. Background technique [0002] Gelatin is widely used in various industries such as medicine, food and health products, and is often used in the preparation of hard capsules and soft capsules; it is also widely used in various food processing industries because it is rich in various amino acids and collagen. The production of gelatin often uses animal skins and bones, undergoes complex physical and chemical treatments, and then undergoes hydrolysis to obtain fat-free and high-protein gelatin products. As broad-spectrum antibiotics, nitrofurans have been widely used in cattle, pigs and other livestock and poultry and aquaculture industries. Since raw materials for gelatin preparation mainly come from animals, the detection of nitrofuran drug residues in gelatin has become an unavoidable problem for manufacturers. Studies have shown that nitrofuran drugs have...

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 靳贵英林生文郑传奇刘亚雄
Owner 广东省药品检验所
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