Bacillus amyloliquefaciens SXZ-N2 strain producing huperzine A and huperzine B and usage

A technology of amylolytic spores, SXZ-N2, applied in the field of microorganisms, can solve the problems of resource scarcity, slow growth, low content of huperzine A, etc., and achieve the effect of low fermentation cost

Active Publication Date: 2017-12-29
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the scarcity of Huperzia serrata plant resources, slow growth, and low content of huperzine A, only relying on the extraction of huperzine A from Huperzia serrata cannot meet the market demand. However, the activity of chemically synthesized huperzine A is far lower. It is better than natural huperzine

Method used

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  • Bacillus amyloliquefaciens SXZ-N2 strain producing huperzine A and huperzine B and usage
  • Bacillus amyloliquefaciens SXZ-N2 strain producing huperzine A and huperzine B and usage
  • Bacillus amyloliquefaciens SXZ-N2 strain producing huperzine A and huperzine B and usage

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Embodiment Construction

[0012] The present invention will be described in detail below with reference to specific embodiments, but the protection scope of the present invention is not limited to the following embodiments.

[0013] Isolation and culture of strains

[0014] In this example, after the Huperzia serrata material was rinsed with clean water, it was then ultrasonicated with clean water for 10 minutes to separate its leaves and stems and cut them into small segments of about 4 cm, soaked in 0.1% mercuric chloride for 4 minutes, and then soaked in 70% ethanol for 2 minutes, Soak in 5% sodium hypochlorite for 5 minutes, then rinse with sterile water for several times, grind it into a homogenate under aseptic conditions, and spread 200 μl of the homogenate on each antibiotic-free PDA plate, and incubate at 30°C for 1 -2d. During the culturing process, 20 colonies were randomly selected according to the bacterial growth, and then transferred to a new LA medium. After several times of plate stre...

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Abstract

The invention provides a bacillus amyloliquefaciens SXZ-N2 strain producing huperzine A and huperzine B. The strain can produce huperzine A and huperzine B. The strain is named as SXZ-N2 and belongs to bacillus amyloliquefaciens. The strain is collected in China Center for Type Culture Collection (CCTCC) on September 14, 2017 in Wuhan University, Wuhan, China, with a postal code of 430072 and a collection number of CCTCC NO: M2017510. A 16S rDNA sequence of the strain is shown as SEQ ID (sequence identifier) NO. 1. The bacillus amyloliquefaciens SXZ-N2 strain can be used for fermentation preparation of huperzine A and huperzine B. Microbial fermentation conditions are as follows: a formula of a culture medium comprises 5g/L yeast powder, 6g/L peptone, 5g/L sodium chloride and 8g/L sucrose; a pH (potential of hydrogen) is 8.0, culture conditions are as follows: 32 DEG C, 150r/min, and fermentation culture lasts for 10 days.

Description

technical field [0001] The invention relates to a strain of Bacillus amyloliquefaciens, which can produce Huperzine A and Huperzine B, and belongs to the technical field of microorganisms. Background technique [0002] At present, the research on plant endophytes has gradually received attention, and various endophytes of different plants have been discovered one after another. The diversity of endophyte species also endows the diversity of its metabolites, which also indicates that the use of microbial metabolism to explore new resources such as biology, chemistry, and medicine has great potential. A large number of studies have shown that endophytes can produce the same or similar active substances and precursors as host plants. This discovery directly promotes the extensive research on endophytes, especially medicinal endophytes. With the gradual research on medicinal plant endophytes, the use of endophytes that can metabolize and produce corresponding active ingredients...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/12C12P17/18C12R1/07
CPCC12P17/12C12P17/182C12N1/205C12R2001/07
Inventor 张鹏宋发军张学智刘盼李浩东张宏庆黄鑫林爱华
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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