Trace identification method for natural Russian musk and natural forest musk

An identification method, a technology of musk muskrat, applied in the field of trace identification of natural Russian musk and natural musk musk, can solve the problems of high professional level and practical experience requirements, reduced drug efficacy, low value, etc., to achieve professional level and Low practical experience requirements, the effect of suppressing the loss of volatile components, and improving accuracy and reliability

CN107561191AActive Publication Date: 2018-01-09BEIJING FORESTRY UNIVERSITY +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2018-01-09

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Abstract

The invention relates to the fields of pharmaceutical analysis and traditional Chinese medicine identification and particularly relates to a trace identification method for natural Russian musk and natural forest musk. The method comprises a sample pretreatment step and a combined GC-MS chromatographic analysis and data treatment step. GC-MS color spectra of the natural Russian musk and the natural forest musk are contrastively analyzed, and the type of the musk can be determined by virtue of the significant difference of characteristic peaks of muscone in two types of musk and the comparisonof analysis data of the contents of muscone in two types of musk, so that the natural Russian musk and the natural forest musk can be conveniently, rapidly and accurately analyzed and judged. The operation specification and conditions of the method are specific, and the influence caused by the subjective factor of people is low, so that the accuracy and reliability of a result are remarkably improved; and furthermore, the method is simple, convenient and feasible, low in requirements on the professional level and practical experience of an operator and is vey suitable for being popularized andused in the field of pharmaceutical detection.
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Description

technical field

[0001] The invention relates to the fields of drug analysis and identification of traditional Chinese medicines, in particular to a micro-identification method for natural Russian musk and natural forest musk. Background technique

[0002] Natural musk is a very precious Chinese herbal medicine. It has the effects of resuscitating the mind, promoting blood circulation and dredging collaterals, reducing swelling and relieving pain, etc. It has a long medicinal history of more than 2,000 years, and has played a long-term and important role in the history of medical care of the Chinese nation. Not only that, natural musk can also be used to make high-grade spices.

[0003] Musk is produced in the sac glands of male musk deer, which is the dry secretion in the mature male musk sac, and forest musk is mainly used in traditional Chinese medicine. Forest musk deer is a national first-class protected animal. With the reduction of the number of wild forest musk deer ...

Examples

Embodiment

[0051] A trace identification method of natural Russian musk and natural forest musk, comprising the following steps:

[0052] (1) Sample pretreatment: freeze-dry the natural Russian musk to be detected with a freeze dryer precooled to -50°C for 18 hours to obtain a natural Russian musk freeze-dried powder, weigh 0.05 g of the above-mentioned natural Russian musk freeze-dried powder, and use Anhydrous CaSO 4 Absorb and dry for 72 hours, then add 1ml of dichloromethane to extract for 15 minutes, filter and take 100 μl of the extract as the natural Russian musk test sample, and use the same method to process the natural forest musk standard product to obtain the natural forest musk test sample;

[0053] (2) Gas chromatography-mass spectrometry GC-MS chromatographic analysis: the two kinds of test samples obtained in the previous step were respectively injected into a gas spectrometry analyzer for GC-MS analysis, and the chromatographic conditions were as follows:

[0054] Injec...