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InDel marker for analyzing genetic diversity and identifying variety of rice and primer and application

A genetic diversity, molecular marker-assisted technology, applied in the field of molecular biology, can solve the problems of difficulty in distinguishing near-isogenic lines or functional substitution lines, lack of correlation, poor correlation of agronomic traits, etc., to achieve cost control, versatility Good results

Active Publication Date: 2018-02-16
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, due to the poor or lack of correlation between SSR markers and the agronomic traits of varieties, it is difficult to distinguish near-isogenic lines or functional substitution lines. For such varieties, it is necessary to use InDel markers (functional markers) of related characteristic functional genes to Differentiate from the original species

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  • InDel marker for analyzing genetic diversity and identifying variety of rice and primer and application
  • InDel marker for analyzing genetic diversity and identifying variety of rice and primer and application
  • InDel marker for analyzing genetic diversity and identifying variety of rice and primer and application

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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with specific examples, but should not be construed as a limitation of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0022] 1 Test materials and methods

[0023] 245 samples of the main hybrid rice and its parents in the middle and lower reaches of the Yangtze River were collected (Table 2), including 157 samples of conventional species / parents, 38 samples of two-line hybrids, and 50 samples of three-line hybrids. Some of the samples with questionable purity were planted in the field to remove impurities, and the young leaves of typical individual plants (10 individual plants for each variety) were selected at the heading stage, and the remaining samples were sent out indoors; the DNA...

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Abstract

The invention belongs to the technical field of biologics and in particular discloses an InDel marker for analyzing genetic diversity and identifying varieties of rice, a locus of the InDel marker inrice, a primer for acquiring the InDel marker, and related application. The InDel marker is good in universality and needs no large-size equipment or instrument; by adopting the InDel marker disclosedby the invention, genotypes of varieties to be tested and genetic differences of varieties can be precisely distinguished, and differences of loci in very close genetic distances can be also distinguished; and after a primer of one locus is designed, a plurality of individuals of the locus can be specifically studied, and the cost can be very well controlled.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to an InDel marker for analyzing rice genetic diversity and identifying varieties, primers and applications. Background technique [0002] Rice is an important food crop in my country, and hybrid rice has made great contributions to food security in my country and the world with its remarkable yield advantage. The promotion and use of new varieties has played an extremely important role in ensuring my country's food supply and meeting people's diverse needs. From 2001 to 2015, 6,310 rice varieties passed provincial-level and above approvals across the country. Effectively distinguishing and identifying these variety materials has important practical significance for rice variety breeding, testing, quality and market management, and property rights protection. [0003] Varieties of morphological differences and differences in characteristics, in essence, are ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 许学秦瑞英马卉倪金龙李莉汪秀峰李浩杨亚春宋风顺王钰杨剑波
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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