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Oxidized andrographolide, preparation method and application in enhancing of in-vivo killing capacity of CIK (cytokine induced killer) cells

A technology of andrographolide and seeds, which is applied in the field of chemistry and can solve the problems of oxidized andrographolide chemical structure, preparation method and pharmacological action that have not been reported.

Active Publication Date: 2018-03-23
上海陶术生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The chemical structure, preparation method and pharmacological action of the oxidized andrographolide have not been reported

Method used

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  • Oxidized andrographolide, preparation method and application in enhancing of in-vivo killing capacity of CIK (cytokine induced killer) cells
  • Oxidized andrographolide, preparation method and application in enhancing of in-vivo killing capacity of CIK (cytokine induced killer) cells
  • Oxidized andrographolide, preparation method and application in enhancing of in-vivo killing capacity of CIK (cytokine induced killer) cells

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of embodiment 1 oxidized andrographolide (quaternary phosphine-1)

[0039] Step S1, strain cultivation:

[0040] Inoculate C. brevis strains on fresh slant medium and culture at a constant temperature of 25°C for 3 days; transfer the bacteria from the slant medium to an Erlenmeyer flask filled with liquid medium, and culture with shaking at 25°C and 135r / min After 2 days, it will become the seed solution; absorb the seed solution and transfer it to the Erlenmeyer flask containing the fermentation medium, shake it at 25°C and 135r / min for 2 days, and then become the culture solution;

[0041] Wherein, the formula of the liquid medium is: peeled and boiled mashed potatoes, 200g / L; potassium dihydrogen phosphate, 3g / L; magnesium sulfate, 0.75g / L; glucose, 20g / L; vitamin B1, 0.15g / L; adjust the pH value to 6.0;

[0042] The fermentation medium is to add 50 μM quaternary phosphine ionic liquid coded as quaternary phosphine-1 to the above liquid medium;

...

Embodiment 2

[0055] The preparation of embodiment 2 oxidized andrographolide (quaternary phosphine-2)

[0056] The only difference between this example and Example 1 is that quaternary phosphine-2 is used instead of quaternary phosphine-1. Specifically include the following steps:

[0057] Step S1, strain cultivation:

[0058] Inoculate C. brevis strains on fresh slant medium and culture at a constant temperature of 25°C for 3 days; transfer the bacteria from the slant medium to an Erlenmeyer flask filled with liquid medium, and culture with shaking at 25°C and 135r / min After 2 days, it will become the seed solution; absorb the seed solution and transfer it to the Erlenmeyer flask containing the fermentation medium, shake it at 25°C and 135r / min for 2 days, and then become the culture solution;

[0059] Wherein, the formula of the liquid medium is: peeled and boiled mashed potatoes, 200g / L; potassium dihydrogen phosphate, 3g / L; magnesium sulfate, 0.75g / L; glucose, 20g / L; vitamin B1, 0.15g ...

Embodiment 3

[0070] The preparation of embodiment 3 oxidized andrographolide (quaternary phosphine-3)

[0071] The only difference between this example and Example 1 is that quaternary phosphine-3 is used instead of quaternary phosphine-1. Specifically include the following steps:

[0072] Step S1, strain cultivation:

[0073] Inoculate C. brevis strains on fresh slant medium and culture at a constant temperature of 25°C for 3 days; transfer the bacteria from the slant medium to an Erlenmeyer flask filled with liquid medium, and culture with shaking at 25°C and 135r / min After 2 days, it will become the seed solution; absorb the seed solution and transfer it to the Erlenmeyer flask containing the fermentation medium, shake it at 25°C and 135r / min for 2 days, and then become the culture solution;

[0074] Wherein, the formula of the liquid medium is: peeled and boiled mashed potatoes, 200g / L; potassium dihydrogen phosphate, 3g / L; magnesium sulfate, 0.75g / L; glucose, 20g / L; vitamin B1, 0.15...

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Abstract

The invention discloses oxidized andrographolide, a preparation method and application in enhancing of in-vivo killing capacity of CIK (cytokine induced killer) cells. The oxidized andrographolide isa product of which the hydroxyl at the C18 site of the andrographolide is oxidized. The preparation method comprises the following steps of S1, culturing of bacterial strain; S2, converting of microorganisms; S3, extracting of converted product; S4, high-speed reverse purifying, wherein the ion liquid with effective concentration is added into the fermenting culture medium in step S1. The invention discloses an andrographolide derivative, and the andrographolide derivative is an oxidized product of the andrographolide; the andrographolide derivative is prepared by a microorganism conversion method. The oxidized andrographolide can enhance the in-vivo killing capacity of CIK cells on tumors.

Description

technical field [0001] The invention belongs to the field of chemistry, and relates to oxidized andrographolide, a preparation method and an application for enhancing the lethality of CIK cells in vivo. Background technique [0002] When the applicant prepared dehydroandrographolide by biological method (submitted patents 2017109643698, 2017109607475, 201710964407X), a kind of oxidized andrographolide was isolated from the microbial transformation product, and the oxidized andrographolide could enhance the anti-cancer effect of CIK cells in vivo. Lethality. [0003] The chemical structure, preparation method and pharmacological action of the oxidized andrographolide have not been reported. Contents of the invention [0004] The purpose of the present invention is to provide an oxidized andrographolide, a preparation method and an application for enhancing the lethality of CIK cells in vivo. [0005] The present invention is realized by following technical scheme: [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/60C12P17/04C12N5/0783A61P35/00C12R1/645
CPCC07D307/60C12N5/0638C12N2501/999C12P17/04
Inventor 王严薛紫彤樊星
Owner 上海陶术生物科技有限公司
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