Purpose of rhapontin in preparation of medicine for treating insulin-resistant relevant diseases

A technology of insulin resistance and rhein, applied in metabolic diseases, endocrine system diseases, cardiovascular system diseases, etc., can solve the problems of unclear mechanism of action of functional compounds, few research reports on monomeric compounds, etc., and achieve inhibition of lipid droplet accumulation. , reduce the transcription level and promote the effect of

Inactive Publication Date: 2018-04-06
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Abstract
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Problems solved by technology

However, the current research results are all based on the fenugreek extract or extraction parts, and there are still few research reports on its monomeric compounds, and its specific functional compounds and their mechanism of action are still unclear.

Method used

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  • Purpose of rhapontin in preparation of medicine for treating insulin-resistant relevant diseases
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  • Purpose of rhapontin in preparation of medicine for treating insulin-resistant relevant diseases

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Embodiment Construction

[0047] Orientin, isorientin, vitexin and isovitexin are 4 kinds of flavonoids, and terratin, deoxyrheidin and emodin are 3 kinds of stilbenes.

[0048] 1. Effects of fenugreek compounds on the differentiation of 3T3-L1 cells

[0049] (1) Effects of fenugreek compounds on the viability of 3T3-L1 preadipocytes

[0050] The viability of 7 fenugreek compounds, orientin, isorientin, vitexin, isovitexin, terretin, deoxyrhein and emodin, on 3T3-L1 preadipocytes was determined by MTT assay Impact. 3T3-L1 preadipocytes were seeded into 96-well culture plates, 1×10 per well 4 Cells (150 μL culture medium) were cultured with DMEM medium containing 10% fetal bovine serum for 12 hours until the cells were completely confluent, and replaced with serum-free medium prepared with concentrations of 0, 0.1, 1, 10, 50, and 100 μM respectively. The cells were treated with 150 μL of the compound for 48 hours, and 4 replicate wells were set for each concentration. After the incubation, add 10 μL...

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Abstract

The invention discloses a purpose of rhapontin in preparation of a medicine for treating insulin-resistant relevant diseases. Seven kinds of compounds are extracted from fenugreek; the several kinds of compounds are subjected to activity study; 3T3-L1 cells are used for building an insulin resistance (IR) module; the blood sugar lowering activity of the compound is further subjected to deep studyfrom the mitochondrion dysfunction level; the prevention and treatment mechanism of the seven compounds on diabetes mellitus is discussed. The result shows that the compound can inhibit the 3T3-L1 cell differentiation; the lipid droplet accumulation is inhibited; the TG content is reduced; the fat factor transcription level can be reduced through relevant signal paths; in addition, by lowering thelevel of ROS in the cells, the compound recovers the 3T3-L1 cell mitochondrion membrane potential; the generation of ATP in the cells is generated; the mitochondrion DNA injury is prevented; the mitochondrion generation is promoted.

Description

technical field [0001] The invention relates to the screening of active compounds, in particular to the use of rhizomatin in the preparation of diseases related to insulin resistance. Background technique [0002] Fenugreek (Trigonella foenum-graecum L.) is a leguminous plant belonging to the genus Fenugreek, an annual herb, also known as fenugreek, vanilla, etc., mainly distributed in China, India, northern Africa and other countries. Fenugreek, as an economic crop with the same source of medicine and food, has a long history of medicinal and edible use, which is recorded in many ancient Chinese pharmacopoeias. "Compendium of Materia Medica" records that fenugreek has a significant effect on treating diseases such as "cold-damp beriberi", "small intestine gas pain", "yin swelling and pain", and "qi attack headache". "Jiayou Materia Medica" records: Fenugreek "mainly controls viscera deficiency and cold air; gets aconite and sulfur to treat kidney deficiency and cold, abdom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7034A61K31/085A61K31/7048A61P5/50A61P3/00A61P3/06A61P3/10A61P9/00A61P9/12
CPCA61K31/7034A61K31/085A61K31/7048
Inventor 王洪伦栾广祥铁芳芳索有瑞
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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