Compositions and methods for DNA and RNA extraction from tissue samples

Abstract

Methods and reagents are provided for the rapid extraction of nucleic acids from a cell or tissue sample. In certain embodiments the sample comprises a formalin fixed paraffin embedded sample (e.g., aFFPET sample), or a fine needle aspirate and / or a cell / tissue smear. In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 DEG C to about 100 DEG C; and recovering the nucleic acid from said lysis solution.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of and priority to USSN 62 / 196,774, filed July 24, 2015, which is hereby incorporated by reference in its entirety for all purposes. [0003] Statement of Government Funding [0004] [Not applicable] Background technique [0005] The use of gene expression profiling is not only popular in a variety of research applications, but is rapidly becoming part of many treatment options. For example, determining gene expression levels in tissues is important for precise diagnosis of human disease and is increasingly used to determine the course of treatment for patients. Pharmacogenomic approaches can identify patients who are likely to respond to specific drugs and can lead to new treatments. [0006] For example, thymidylate synthase (TS) is an integrase in DNA biosynthesis where it catalyzes the reductive methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dT...

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