Three-dimensional helical structure cell sorting microfluidic chip and manufacturing method thereof

Abstract

The invention discloses a three-dimensional helical structure cell sorting micro-fluidic chip and a manufacturing method of the three-dimensional helical structure cell sorting micro-fluidic chip. Themicro-fluidic chip is provided with a helical ladder-like extending three-dimensional helical micro-channel, cells arranged in disorder migrate and focus to different balance positions in the micro-channel according to the size through the inertial effect of the fluid on the microscale when an injected sample flows in the micro-channel, and the cells of different sizes are finally sorted, exported and collected through the reasonable design of a sample outlet. In addition, the micro-fluidic chip uses the helical ladder-like extending three-dimensional layout, so that the characteristic of theplanar structure of the traditional micro-fluidic chip is broken through. Multiple cell sorting channels are arranged within the same plane area, so that multiple channels work at the same time, thehandling flux of samples is increased within the unit time, and the rapid sorting and preprocessing of the large-size sample is met.

Description

technical field [0001] The invention relates to microfluidic technology, more specifically, to a three-dimensional helical structure cell sorting microfluidic chip and a manufacturing method thereof. Background technique [0002] Microfluidic technology, as a new technology to realize fluid sample or micro-nano particle detection, analysis, manipulation, synthesis and other functions at the micro-nano scale, has been widely used in clinical medicine due to its advantages of small size, low cost and less sample consumption. , biochemical analysis, biology and other research fields of detection and analysis applications. It has become an important enabling technology in microfluidic research to replace expensive traditional cabinet-type diagnostic analysis equipment and realize the efficient transportation, sorting, extraction, assembly and mixing of micro-nano materials. How to achieve efficient sorting of micro-scale objects has very important practical value for the develo...

AI Technical Summary

Problems solved by technology

However, these methods have their own shortcomings, such as energy consumption in the field, high cost and complicated operation.

In addition, due to the low flow rate in most microfluidic chips, the sorting efficiency is greatly reduced, which cannot meet the needs of rapid sorting and processing of large-volume samples.

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