One for detection of fe 3+ The organic fluorescent sensing probe of the present invention and its preparation method, use method and application

Abstract

The invention discloses an organic fluorescent sensation probe for detecting Fe<3+>. A preparation method of the organic fluorescent sensation probe comprises the following steps: mixing an oily fluorescent sensation material with formic acid, and performing backflowing for 1 hour at 40-90 DEG C so as to obtain raw material probe molecules with protected groups; mixing the raw material probe molecules with tri-halogen phenol, and performing backflowing for 24 hours at 55-110 DEG C with a catalyst, thereby obtaining the organic fluorescent sensation probe. The organic fluorescent sensation probe is good in water solubility, good in biocompatibility and wide in inspection range, the advantages that an organic fluorescent probe is good in material specificity and high in sensitivity are maintained, the lower detection limit of iron ions can be greatly increased, and the lower detection limit of the probe is at a world leading level.

Description

technical field [0001] The present invention relates to Fe 3+ The field of ion detection, especially relates to a kind of for detecting Fe 3+ The organic fluorescent sensing probe and its preparation method, use method and application. Background technique [0002] Fe 3+ Ions are widely distributed in natural water, vegetables, fruits, crops and animals. In the process of metabolism, Fe 3+ Ions are involved in many very important biochemical processes, however, the organism itself takes too much or too little Fe 3+ Ions can produce adverse reactions. Great efforts have been devoted to the qualitative or quantitative Fe 3+ Ion determination, along with the development of sensing technology, has developed and applied a variety of methods, including atomic absorption, chromatography, electrochemical method, flow injection method and fluorescent sensing materials, which can be applied to Fe 3+ Ion detection, but these methods not only have high detection costs, complex de...

AI Technical Summary

Problems solved by technology

However, most of the organic probe molecules are synthesized in the oil phase, and the molecules are overall hydrophobic, and the poor water solubility has always been the status quo that the organic fluorescent ion detection method cannot break through. At present, the lowest detection limit in the world is 2pmol / ml

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