Double-antibody combined fluorescence detection kit for liver cancer resection postoperative prognosis

A fluorescent detection and double-antibody technology, applied in measuring devices, instruments, disease diagnosis, etc., can solve problems such as difficulty in distinguishing the expression of tumor cells and immune cells, limited curative effect judgment, etc., and achieve mature test methods, easy repeatability, and detection process easy effect

Inactive Publication Date: 2018-08-14
SUN YAT SEN UNIV CANCER CENT
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Problems solved by technology

However, traditional immunohistochemical staining is used to evaluate the expression of PD-L1 clinically, and it is difficult to distinguish the expression of tumor cells and immune cells, so the ability to judge the efficacy is limited

Method used

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  • Double-antibody combined fluorescence detection kit for liver cancer resection postoperative prognosis
  • Double-antibody combined fluorescence detection kit for liver cancer resection postoperative prognosis

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Embodiment Construction

[0016] The double-antibody combined fluorescence detection kit for prognosis after liver cancer resection according to the present invention includes a first antibody and a second antibody, and the first antibody includes an antibody for detecting the following two protein molecular markers: CD68 (Cell Differentiation Antigen 68), PD-L1 (Programmed Death Ligand 1); also includes TSA (Tyramide Signal Amplifying Reagent). The secondary antibody is a horseradish peroxidase-labeled secondary antibody.

[0017] For the double-antibody combined fluorescence detection kit described in the present invention, its effectiveness is tested through the following steps.

[0018] (1) Specimen testing

[0019] 1. Select the paraffin section of liver cancer that includes the tumor area, and ensure that there is no large necrosis;

[0020] 2. Obtain 8 paraffin sections of 4 μm, bake the slices at 60°C for 2 hours, take them out, and cool slightly;

[0021] 3. Dewax with xylene twice at room ...

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Abstract

The invention relates to a double-antibody combined fluorescence detection kit for liver cancer resection postoperative prognosis. The double-antibody combined fluorescence detection kit for liver cancer resection postoperative prognosis comprises a first antibody and a second antibody, wherein the first antibody is selected from the antibody combination of the following protein molecular markers:CD68 (cell differentiation antigen 68) and PD-L1 (procedural death ligand 1). The kit uses double-antibody combination indexes; the expression mode of the PD-L1 in tissues can be precisely reflected,so that the prognosis of a patient is better evaluated; the kit can help doctors to select the proper treatment user groups and modes, so that the powerful support is provided for effective diagnosisand treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a double-antibody combined fluorescence detection kit for prognosis after liver cancer resection, in particular to a double-antibody combined fluorescence detection kit for detecting PD-L1 molecular expression patterns in liver cancer tissues . Background technique [0002] Primary hepatocellular carcinoma (hereafter referred to as liver cancer) is one of the most common malignant tumors with the worst prognosis in my country, and its morbidity and mortality rank among the forefront of malignant tumors. At present, the most effective methods for the treatment of liver cancer are still surgical resection and liver transplantation; however, most patients are already in the middle and advanced stages when they are diagnosed, and do not have the indications and conditions for surgery. Survival rates are still low. A large number of clinical trials in recent years have proved that immunot...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/533
CPCG01N33/533G01N33/57438G01N2800/085
Inventor 郑利民陈敏山许静刘超群张耀军石明
Owner SUN YAT SEN UNIV CANCER CENT
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