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Immunofluorescence detection kit for evaluating therapeutic effect of liver cancer patients

An immunofluorescence detection and treatment effect technology, applied in the biological field, can solve the problems of waste of medical resources, delayed disease development, economic loss, etc., and achieve the effects of easy repetition, mature test methods, and simple detection process.

Inactive Publication Date: 2018-02-27
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sorafenib is the world's first anti-angiogenic drug approved for the treatment of liver cancer, and it is also the only molecularly targeted drug that has been proven to prolong the survival of liver cancer patients. However, the disease control rate of sorafenib in the treatment of liver cancer is only 42%. -62%, the main reason is that there is currently no clear indicator to screen the target population for the drug treatment, and patients can only blindly accept sorafenib treatment, which not only delays the development of the disease for ineffective patients, but also causes huge economic losses and medical treatment. waste of resources
[0003] At present, there are no molecular markers or detection kits that can be used to judge the efficacy of sorafenib treatment in patients with liver cancer

Method used

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  • Immunofluorescence detection kit for evaluating therapeutic effect of liver cancer patients
  • Immunofluorescence detection kit for evaluating therapeutic effect of liver cancer patients

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Preparation of the immunofluorescence detection kit of the present invention

[0014] In the present invention, CD34 antigen is selected as the overall vascular endothelial cell marker in tumor tissue, CXCR4 antigen is used as the vascular endothelial top cell marker, and the vascular endothelial cells with double positive CXCR4 and CD34 antigens are marked by immunofluorescent double staining technology.

[0015] Table 1: Sources of antibodies and specific experimental conditions

[0016] index

Antibody source

Repair method

Antibody dilution ratio

CD34

Zhongshan Golden Bridge, Item No.: ZM-0046

Microwave 20min, 10mM CB (pH6.0)

1:200

CXCR4

Abcam, Cat. No. ab124824

Microwave 20min, 10mM CB (pH6.0)

1:100

[0017] CB: citrate buffer.

[0018] Immunofluorescence detection kit of the present invention is determined by following experiments:

[0019] First, immunofluorescence was used to detect the ...

Embodiment 2

[0023] Embodiment 2: Detection experiment of the immunofluorescence detection kit of the present invention

[0024] For the immunofluorescence detection kit described in the present invention, its effectiveness is tested through the following steps.

[0025] (1) Specimen testing

[0026] 1. Select the paraffin section of liver cancer including the tumor area (before taking sorafenib treatment), and ensure that there is no large necrosis;

[0027] 2. Obtain 8 paraffin sections of 4 μm, bake the slices at 60°C for 2 hours, take them out, and cool slightly;

[0028] 3. Dewax with xylene twice at room temperature, 10 minutes each time;

[0029] 4. Wash off the xylene in 100% ethanol, and then pass through 95% alcohol, 80% alcohol, and 70% alcohol in sequence, each time for 5 minutes;

[0030] 5. Wash in double distilled water for 5 minutes;

[0031] 6. With 0.3% H 2 o 2 Block endogenous peroxidase activity, room temperature for 10 minutes;

[0032] 7. Wash in double distill...

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Abstract

The invention belongs to the technical field of biology, and relates to an immunofluorescence detection kit for evaluating the therapeutic effect of liver cancer patients, in particular to a double-antibody detection kit for evaluating the effect of sorafenib treatment on hepatocellular carcinoma patients. The immunofluorescence detection kit for evaluating the therapeutic effect of the liver cancer patients comprises a first antibody and a second antibody, wherein the first antibody is a combination of antibodies selected from the following protein molecular markers: CD34(cell differentiationantigens 34) and CXCR4 (C-X-C chemokine receptors 4). The kit adopts double-antibody binding judgment indexes, has the characteristics of objectiveness, accuracy, simplicity, intuitiveness, easinessin repetition and the like, can effectively evaluate the therapeutic effect of sorafenib taken by the liver cancer patients, and can be used for screening the liver cancer patients suitable for the sorafenib treatment to better help doctors choose right people to be treated, so as to improve the therapeutic effect and prolong the survival time of the patients.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an immunofluorescence detection kit for evaluating the therapeutic effect of liver cancer patients, in particular to a double antibody detection kit for evaluating the effect of sorafenib treatment on hepatocellular carcinoma patients. Background technique [0002] Hepatocellular carcinoma Liver cancer (referred to as liver cancer) is one of the most common malignant tumors, and the death toll ranks third among malignant tumors in my country. The prognosis of liver cancer is extremely poor, and most patients have lost the chance of surgery at the time of diagnosis, because liver cancer is very prone to intrahepatic or extrahepatic metastasis. Liver cancer is rich in angiogenesis, and hematogenous metastasis is an important reason for easy metastasis and recurrence of liver cancer. Therefore, inhibition of angiogenesis is considered to be an important strategy for the treatment of liver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57438
Inventor 郑利民陈敏山许静梁静徐立孟亚明
Owner SUN YAT SEN UNIV CANCER CENT
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