The invention belongs to the technical field of
tumor vaccines, and discloses a personalized tumor vaccine based on hydrogel and a preparation method and application thereof, the preparation method comprises the following steps: adding a chemotherapeutic
drug into a
mixed solution of
polyphenol and
metal ions, uniformly mixing, adjusting the pH value of the solution, and reacting for 2-8 hours to obtain a
metal polyphenol nano-carrier loaded with the chemotherapeutic
drug; co-culturing the obtained
metal polyphenol nano-carrier loaded with the chemotherapeutic
drug and
tumor cells for 12-48 hours, and inducing the
tumor cells to generate
immunogenic cell death, wherein the concentration of the metal polyphenol nano-carrier loaded with the chemotherapeutic drug is 1-20 [mu] g / mL; the tumor
cell suspension in the ICD state and the STING
agonist solution are mixed with the
sodium alginate solution, and the personalized tumor vaccine is obtained. The ICD is induced
in vitro by the chemotherapeutic drug, so that the toxic and side effects of
systemic administration are reduced, the types of antigens are enriched, and a powerful guarantee is provided for the vaccine to activate a strong in-vivo anti-tumor immune response.