A kind of Aspergillus parasitica spore compound insecticide and preparation method thereof

A technology of Aspergillus parasiticus and Aspergillus spores, which is applied in the field of Aspergillus parasiticus spore compound insecticide and its preparation, which can solve the problems of not being broad-spectrum, limiting the scope of use, and not lasting, so as to avoid adverse effects and improve the insecticidal effect and insecticidal efficiency, the effect of controlling reproduction

Active Publication Date: 2020-05-26
鹤壁市人元生物技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Botanical pesticides have the advantages of safety, high efficiency, low toxicity, and low residue, which cannot be compared with chemical pesticides. They play a very positive role in solving the problems caused by chemical pesticides, and have become a hot spot in the development of innovative pesticides. However, botanical insecticides, as emerging insecticidal products, still face many problems in order to replace chemical pesticides and be widely used in agricultural production, especially botanical insecticides are highly targeted and do not have a broad spectrum , this is the advantage of botanical pesticides, but it also limits its application in production practice. Or some specific crops, which limit its scope of use, and botanical insecticides also have the disadvantages of short control effect and not lasting; while fungal insecticides have a wide range of hosts, are environmentally safe, and are not effective in controlling pests. The advantages of producing drug resistance have attracted widespread attention in research and application, but many fungi are aimed at the control of one pest and do not have a broad spectrum

Method used

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  • A kind of Aspergillus parasitica spore compound insecticide and preparation method thereof
  • A kind of Aspergillus parasitica spore compound insecticide and preparation method thereof
  • A kind of Aspergillus parasitica spore compound insecticide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The present embodiment provides a compound insecticide for Aspergillus parasitica spores, which is a wettable powder, and by mass percentage, it includes 5.5% calcium lignosulfonate, 2.5% sodium fluorescein, 1.5% oxalic acid, 2.5% % polyethylene glycol, 3% spore germination accelerator, 5% rotenone powder and 80% spore powder (Aspergillus parasiticus RWSF001-02); wherein, the spore germination accelerator includes 0.15% peptone, 0.05% yeast powder, 0.1 % glucose, 0.1% ammonium sulfate, 0.1% dipotassium hydrogen phosphate, 95% diatomaceous earth.

[0024] The preparation method of described Aspergillus parasitica spore compound insecticide comprises the following steps:

[0025] 1) Preparation of Aspergillus parasitica spore powder: transfer the Aspergillus parasitica species to PDA liquid medium, and place it on a shaker and cultivate it at 28°C for 24 hours to make liquid mycelium seeds. The inoculum amount was inoculated into a solid medium composed of cornmeal, bran...

Embodiment 2

[0033] The present embodiment provides a kind of Aspergillus parasitica spore compound insecticide, the difference between the present embodiment and embodiment 1 is: by mass percentage, it comprises 5% calcium lignosulfonate, 2% sodium fluorescein, 1% oxalic acid, 2% polyethylene glycol, 2% spore germination accelerator, 5% rotenone powder and 83% spore powder (Aspergillus parasiticus RWSF001-02); wherein, the spore germination accelerator includes 0.1% peptone, 0.03% yeast powder , 0.05% glucose, 0.05% ammonium sulfate, 0.15% dipotassium hydrogen phosphate, 96.4% diatomaceous earth.

Embodiment 3

[0035]The present embodiment provides a kind of Aspergillus parasitica spore compound insecticide, the difference between the present embodiment and embodiment 1 is: by mass percentage, it comprises 6% calcium lignosulfonate, 3% sodium fluorescein, 2% oxalic acid, 3% polyethylene glycol, 4% spore germination accelerator, 5% rotenone powder and 77% spore powder (Aspergillus parasiticus RWSF001-02); wherein, the spore germination accelerator 0.2% peptone, 0.07% yeast powder, 0.15% glucose, 0.15% ammonium sulfate, 0.15% dipotassium hydrogen phosphate, 97% diatomaceous earth.

[0036] performance verification

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PUM

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Abstract

The invention provides an aspergillus spore composite pesticide. The aspergillus spore composite pesticide comprises the following components by mass percentage: 80-90% of an active component and 10-20% of an auxiliary agent, the active component comprises rotenone powder and aspergillus spore powder with a mass ratio being 1:(15-20), and the spore number of the aspergillus spore powder is 8*108 / g. The aspergillus spore composite pesticide employs combination of rotenone and parasitic aspergillus, an insecticidal effect mechanism of paralysis, parasitism, killing and epidemic performance in order is formed, and breeding of pest population can be effectively controlled. The invention also provides the preparation method of the aspergillus spore composite pesticide.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and in particular relates to a compound insecticide of Aspergillus parasitica spores and a preparation method thereof. Background technique [0002] my country is a large agricultural country, and pests such as insects, acarina (mite and tick), aphids and nematodes are common agricultural pests, and the economic losses caused by pests are huge every year. In order to control pests, various insecticides have been developed. Existing insecticides mainly include chemical insecticides, botanical insecticides and fungal insecticides. [0003] Among them, chemical pesticides are used to control pests, and the killing effect is very significant in a short period of time, but excessive use of chemical pesticides leaves residues in soil, water and air, and also has negative effects on non-target organisms and ecological balance. Negative Effects. In addition, pests readily develop resistance to che...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/34A01N43/90A01P7/04A01P7/02
CPCA01N43/90A01N63/30
Inventor 王树伟李静王建红金丽崔金杰马艳
Owner 鹤壁市人元生物技术发展有限公司
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