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Application of licochalcone A in preparation of medicine for resisting porcine epidemic diarrhea virus

A technology for porcine epidemic diarrhea and licorice chalcone, applied in the direction of antiviral agents, ketone active ingredients, etc., can solve the problem that virus particles do not have hemagglutination, and achieve easy absorption, high biological metabolism rate, and low residue. Effect

Active Publication Date: 2018-09-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, PEDV is sensitive to fat-soluble fluids, and the virion does not have hemagglutination

Method used

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  • Application of licochalcone A in preparation of medicine for resisting porcine epidemic diarrhea virus
  • Application of licochalcone A in preparation of medicine for resisting porcine epidemic diarrhea virus
  • Application of licochalcone A in preparation of medicine for resisting porcine epidemic diarrhea virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Determination of Cytotoxicity of Embodiment 1 Licochalcone A on Vero-E6 Cells

[0028] After counting Vero-E6 cells, they were diluted with 8% fetal bovine serum (FBS) DMEM nutrient solution to an appropriate density and then diluted to 1.5×10 4 The concentration per well was added to a 96-well plate and placed at 37°C, 5% CO 2 After the cells adhered to form a monolayer in the incubator (about 18-20h), dilute licochalcone A with DMEM nutrient solution to: 1 μM, 2 μM, 5 μM, 10 μM, and set 3 sets of repetitions for each concentration. Treat Vero-E6 cells with 100 μl / well of DMSO control and diluted licochalcone A samples on a 96-well cell culture plate for 72 hours, remove the supernatant, add 90 μl of fresh culture medium, and then add 10 μl of MTT solution, and continue to incubate for 4 hours ; Remove the supernatant, add 110 μl Formazan solution to each well, place on a shaker and shake at low speed for 10 minutes to fully dissolve the crystals. Measure the absor...

Embodiment 2

[0030] Example 2 Western Blot Determination of Licochalcone A Inhibiting Porcine Epidemic Diarrhea on Vero-E6 Cells Activity of virus infection

[0031] After Vero-E6 cells were counted, they were diluted with 8% fetal bovine serum (FBS) DMEM nutrient solution to an appropriate density and then diluted to 7×10 5 The concentration per well was added to a 6-well plate and placed at 37°C, 5% CO 2 After the cells adhered to form a monolayer in the incubator and grew to a density of 70-80% (about 18-20h), the licochalcone A was diluted with DMEM nutrient solution to: 1 μM, 2 μM, 5 μM, 10 μM. After pre-treating Vero-E6 cells with DMSO control and different concentrations of licochalcone A at 37°C for 2 hours, they were infected with porcine epidemic diarrhea virus HLJBY strain (MOI=0.01), and placed at 37°C, 5% CO 2 Place in the incubator for 1 hour, wash three times with PBS, add 1ml of DMEM maintenance solution containing 2% fetal bovine serum (FBS) and contain licochalcone A...

Embodiment 3

[0032] Example 3 Fluorescence Quantitative Detection of Licochalcone A Inhibits Porcine Epidemic Diarrhea Virus Infection on Vero-E6 Cells Activity

[0033] After Vero-E6 cells were counted, they were diluted with 8% fetal bovine serum (FBS) DMEM nutrient solution to an appropriate density and then diluted to 7×10 5 The concentration per well was added to a 6-well plate and placed at 37°C, 5% CO 2After the cells adhered to form a monolayer in the incubator and grew to a density of 70-80% (about 18-20 hours), licochalcone A was diluted to 10 μM with DMEM nutrient solution. After pre-treating Vero-E6 cells with DMSO control and licochalcone A at 37°C for 2 hours, they were infected with porcine epidemic diarrhea virus HLJBY strain (MOI=0.01), and placed at 37°C, 5% CO 2 Place in the incubator for 1 hour, wash three times with PBS, add 1ml of DMEM maintenance solution containing 2% fetal bovine serum (FBS) and contain licochalcone A, place at 37°C, 5% CO 2 After culturing i...

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Abstract

The invention discloses application of licochalcone A in preparation of a medicine for resisting porcine epidemic diarrhea virus, wherein the mass percentage content of the licochalcone A is not lowerthan 97.65%, and the application dosage is 1-10 micrometers per liter; the licochalocone A has remarkable effect on resisting porcine epidemic diarrhea virus, is safe and with less toxic and side effects, and has low drug residue and no pollution.

Description

technical field [0001] The invention belongs to the field of antiviral applications, and in particular relates to the use of licochalcone A in the preparation of anti-porcine epidemic diarrhea virus drugs. Background technique [0002] Porcine epidemic diarrhea is a disease caused by porcine epidemic diarrhea virus (PEDV). It is clinically characterized by vomiting, severe diarrhea and dehydration. The disease often occurs in outbreaks in winter and can affect pigs of all ages Infect. Among them, suckling piglets are the most susceptible, the incidence rate can reach 100%, and the case fatality rate reaches 80%-100%. Back-up pigs, sows or fattening pigs are transient diarrhea, and the incidence rate is lower. PEDV first appeared in Europe in the 1970s. However, since 2010, the outbreak of PEDV mutant strains has caused huge economic losses to the pig industry in China, Japan, South Korea, Thailand, the Philippines and other Asian countries. Pandemic in the United States, G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P31/14
CPCA61K31/12A61P31/14
Inventor 钱莺娟郑龙三陈新薄宗义
Owner NANJING AGRICULTURAL UNIVERSITY
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