Cell penetrating cyanine-coupled antibodies
A cell and antibody technology, applied in antineoplastic drugs, anti-infective drugs, allergic diseases, etc., can solve problems such as labor-consuming, damage to the structural and functional integrity of cells or antibodies
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Embodiment approach P1
[0277] Embodiment P1. A cell penetrating conjugate having the formula:
[0278]
[0279] in
[0280] Ar 1 is substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted fused ring aryl or substituted or unsubstituted fused ring hetero Aryl;
[0281] Ar 2 is a substituted or unsubstituted cycloalkylene, a substituted or unsubstituted heterocycloalkylene, a substituted or unsubstituted arylene, or a substituted or unsubstituted heterocyclene Aryl;
[0282] L 1 is a substituted or unsubstituted alkylene or a substituted or unsubstituted heteroalkylene;
[0283] P is a non-cell penetrating protein;
[0284] n is 1 or 2; and
[0285] z 1 and z 2 are independently 1 or 2.
[0286] Embodiment P2. The cell penetrating conjugate of embodiment P1, wherein Ar 1 is a substituted or unsubstituted fused ring aryl or a substituted or unsubstituted fused ring heteroaryl; and Ar 2 is a substituted or unsubstituted fused r...
Embodiment approach P3
[0287] Embodiment P3. The cell penetrating conjugate of Embodiment P1 or P2, wherein the cell penetrating conjugate has the formula:
[0288]
[0289] in
[0290] R 1 , R 2 , R 3 and R 4 independently hydrogen, halogen, CX 3 , -CN, -C(O)OH, -CH 2 C(O)OH, -C(O)NH 2 , -OH, -SH, -NO 2 , -NH 2 , -NHNH 2 , -ONH 2 , -NHC=(O)NHNH 2 , substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted substituted or unsubstituted aryl or substituted or unsubstituted heteroaryl; and
[0291] X is -F, -Cl, -Br or -I.
[0292] Embodiment P4. The cell penetrating conjugate of embodiment P3, wherein R 1 , R 2 , R 3 and R 4 are independently hydrogen or substituted or unsubstituted alkyl.
[0293] Embodiment P5. The cell penetrating conjugate of embodiment P3 or P4, wherein R 1 , R 2 , R 3 and R 4 are independently hydrogen or methyl.
Embodiment approach P6
[0294] Embodiment P6. The cell penetrating conjugate of any one of embodiments P3-P5, wherein R 1 , R 2 and R 3 is methyl.
PUM
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