A biomarker panel for Takayasu arteritis detection and use thereof
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A biomarker, Takayasu arteritis technology, used in biological testing, material testing products, etc.
Active Publication Date: 2020-12-22
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
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At present, there are no serum antibody markers for TA available clinically. It is urgent to comprehensively and systematically study the overall picture of antibody markers for TA. Finding TA markers for diagnosis and treatment is the current research focus of TA and an urgent clinical problem to be solved.
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Embodiment 1
[0025] Example 1 High-density protein chip screening for TA-related autoantibodies
[0026] Forty TA patient sera, 15 autoimmune disease control sera, and 20 healthy control sera were selected for hybridization with 75 high-density protein chips (CDI, US), and candidate TA-related autoantigens were identified by signal acquisition and data analysis.
[0027] The chip hybridization method was strictly in accordance with the procedure provided by the chip manufacturer, and the chip after hybridization was scanned with a GenePix4000B chip scanner to obtain a TIFF format image ( figure 1 ). According to the operation manual of GenePix Pro 6.0 software, according to the high-throughput chip loading matrix parameters, the software automatically completes the signal pixel segmentation of each protein spot, and at the same time manually checks the signal intensity of all protein spots on the chip one by one, and manually adjusts to avoid impurities. The position and size of the prote...
Embodiment 2
[0032] Example 2 Construction of TA-related autoantibody target antigen protein chip and verification of serum screening
[0033] Due to differences in genetic background and environmental factors in patients with autoimmune diseases, the positive rate of autoantibodies produced by different patients varies greatly. In order to confirm whether the TA-related autoantibodies screened by high-density protein chips in small samples have clinical significance and application value, as well as the sensitivity and specificity of these antibodies for the diagnosis of TA patients, the TA-related autoantibodies screened out by high-density protein chips were analyzed in this study. Related autoantibody target antigens were prepared into TA-related autoantibody target antigen protein chips for clinical expansion sample verification.
[0034] The TA-related autoantibody target antigen protein chip was used for clinical large-scale verification. The serum samples tested included 106 cases ...
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Abstract
The invention discloses a biomarker panel for TA (Takayasu arteritis) detection and an application of the biomarker panel to preparation of reagents for TA diagnosis. The biomarker panel totally contains 22 biomarkers, wherein a first group contains 13 target antigens which are used for differential diagnosis of TA and non-TA by detecting autoantibody level of SPATA7, IL17RB, ZFAND4, PRH2, CLPTM1,PGPEP1, DIXDC1, NDP, SLC27A2, PLA2G16, MAPK1IP1L, HSF2 and NOL3 in serum of a patient, accuracy is 83.6%, and sensitivity and specificity are 50.9% and 94.4% respectively; a second group contains 13target antigens which are used for distinguishing active phase from stationary phase of TA by detecting antibody level of QDPR, PKD1L2, ZFAND4, DIXDC1, NDP, PLA2G16, CIDEA, TRIM36, EIF3H, USF2, AGGF1,SPANXN2 and MCM3 in serum of a patient with TA, accuracy is 96.2%, and sensitivity and specificity are 96.19% and 96.4% respectively.
Description
technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a biomarker for detecting Takayasu arteritis and its application. Background technique [0002] Takayasu arteritis (TA) is a chronic granulomatous large vasculitis, mainly involving the aorta and its branches such as the carotid artery and subclavian artery, causing thickening of the vessel wall and leading to lumen stenosis, occlusion, dilation or aneurysm form. Takayasu arteritis is more common in Asia, mostly in young women, the incidence ratio of male to female is about 1:8-9, the peak incidence of female is around 20 years old, about 90% of the onset is before the age of 30, and the onset is rare after the age of 40 , while men do not have an accurate peak age of onset. The etiology of Takayasu arteritis is still unclear so far, and it may be related to factors such as immune damage caused by infection or genetic factors. The initial onset of TA patients is...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 李永哲胡朝军吴琳温晓婷张奉春
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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