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Application of MTHFD1L (methylenetetrahydrofolate dehydrogenase (NADP+dependent)1like) inhibitor to preparation of tongue squamous cell carcinoma treatment medicament

A drug for the treatment of tongue squamous cell carcinoma, which is applied in the field of MTHFD1L inhibitor and the preparation of a therapeutic drug for tongue squamous cell carcinoma, can solve the problem of no clear report, etc., and achieve the effects of promoting apoptosis and inhibiting the proliferation of tongue squamous cell carcinoma cells.

Active Publication Date: 2018-10-16
XUZHOU CENT HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have shown that some mutants of MTHFD1L are related to neural tube defects, but the specific mechanism of MTHFD1L in the process of tumor proliferation and apoptosis has not been clearly reported yet.

Method used

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  • Application of MTHFD1L (methylenetetrahydrofolate dehydrogenase (NADP+dependent)1like) inhibitor to preparation of tongue squamous cell carcinoma treatment medicament
  • Application of MTHFD1L (methylenetetrahydrofolate dehydrogenase (NADP+dependent)1like) inhibitor to preparation of tongue squamous cell carcinoma treatment medicament
  • Application of MTHFD1L (methylenetetrahydrofolate dehydrogenase (NADP+dependent)1like) inhibitor to preparation of tongue squamous cell carcinoma treatment medicament

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Experimental program
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Embodiment 1

[0093] (1) Experimental method

[0094] 1. Preparation of interfering lentivirus targeting MTHFD1L gene

[0095] For the MTHFD1L gene, a specific target interference sequence was designed, and the shRNA for MTHFD1L was denoted as shMTHFD1L, and the target sequence for the shMTHFD1L was shown in SEQ ID NO.1, specifically:

[0096] 5'-ATTCCAGTTCCTGTATGAT-3' (psc-1, psc28333). The sequence of the siRNA corresponding to shMTHFD1L is shown in SEQ ID NO.2, specifically: 5'-AUUCCAGUUCCUGUAUGAU-3' The sequence of shMTHFD1L itself is shown in SEQ ID NO.3, specifically:

[0097] 5'-ATTCCAGTTCCTGTATGATCTCGAGATCATACAGGAACTGGAAT-3'. The target sequence of the negative control (shCtrl) is shown in SEQ ID NO.4, specifically: 5'-TTCTCCGAACGTGTCACGT-3'.

[0098] Design the shRNA interference sequence according to the target sequence, construct it into the Hu6-MCS-CMV-EGFP plasmid vector, and co-transfect 293T cells with the pHelper 1.0 vector and pHelper 2.0 vector plasmid, and obtain unpur...

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Abstract

The invention belongs to the field of the biopharmaceutical research, and particularly relates to application of a MTHFD1L (methylenetetrahydrofolate dehydrogenase (NADP+dependent)1like) inhibitor. Bywide and deep research, the invention shows that MTHFD1L can be used as a tongue squamous cell carcinoma treatment target for the first time. The MTHFD1L inhibitor can inhibit cell multiplication oftongue squamous cell carcinoma, promote cell apoptosis of tongue squamous cell carcinoma, inhibit cell cloning, inhibit tumorigenesis of tongue squamous cell carcinoma, treat tongue squamous cell carcinoma and develops a new direction for treatment of tongue squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedical research, and in particular relates to the use of MTHFD1L inhibitors in the preparation of drugs for treating tongue squamous cell carcinoma. Background technique [0002] Tongue squamous cell carcinoma (TSCC), referred to as tongue squamous cell carcinoma, is one of the most common malignant tumors in the oral and maxillofacial region. The prognosis is poor. With the advances in surgery and radiotherapy and chemotherapy, the 5-year survival rate of tongue squamous cell carcinoma has increased. However, the overall prognosis remains unfavorable. [0003] The primary site, size, pathological grade, clinical stage and other factors of the tumor can affect the prognosis of the patient. Early prediction of tongue squamous cell carcinoma with a high recurrence rate and lymph node metastasis tendency and the formulation of an effective treatment plan are the key to improving the prognosis of the tongue. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K45/06A61P35/00
CPCA61K45/00A61K45/06A61P35/00
Inventor 孟箭白玉婷韩琨丁紫雪王兴李娜
Owner XUZHOU CENT HOSPITAL
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