a tissue preservation solution

Abstract

The invention discloses a tissue preservation solution. Each 50mL of the tissue preservation solution contains the following components: DMEM / F12 50ml, bFGF 90-110μL, N2 240-260μL, B27 480-520μL, Y-27632 40-60μL, BDNF 400-500ng, NT3 400-500ng. By using the tissue preservation solution of the present invention, the activity of fresh tumor cells can be maintained for a long time, thereby improving the efficiency of obtaining PDX models. Compared with conventional preservation solutions, the tumor formation rate of PDX models using this preservation solution is greatly increased, the tumor utilization rate is greatly improved, and the time for tumor formation and primary cell culture is significantly shortened. Moreover, using this method can enhance the activity of chordoma samples, thereby reducing the cost of chordoma PDX model experiments, enabling more patients to use PDX models for precision medicine of tumors, thereby improving the possibility of chordoma cure.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a fast and efficient optimization method for preparing a tumor transplantation model derived from a patient's chordoma tissue, and specifically relates to a tumor transplantation (Patient- derived xenograft (PDX) model and the method of obtaining high-purity primary tumor cells. Background technique [0002] Chordomas are locally invasive or malignant neoplasms arising from embryonic residual or lost notochord tissue. It can occur anywhere along the midline of the spine, but is most common on the rostral side of the clivus and the sacrum. Chordoma is very destructive in local area, it will endanger the human body because the tumor continues to grow, and it is very easy to relapse after surgery, so it is still a malignant tumor. At present, there is no good drug that can completely cure the tumor. [0003] The PDX tumor model is a new generation of tumor model established b...

AI Technical Summary

Problems solved by technology

Serum can easily differentiate chordoma tumor cells and reduce the ability of malignant proliferation

Therefore, it will lead to the differentiation of tumor samples and the decrease of activity, resulting in a decrease in the success rate of PDX tumor model building, increasing the cost and dosage, and increasing the difficulty of experiments. It is easy to obtain tumor cells contaminated by fibroblasts by culturing tumor tissues in culture medium containing serum. It is difficult to remove fibroblasts in later use, which limits the application of primary tumor cells

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