A Marking Method for Marking the Molecular ID Card of New Hybrid Varieties of Four Seasons Camellia

A technology of molecular ID card and marking method, which is applied in the field of marking the molecular ID card of the new hybrid variety of camellia in four seasons, can solve the problems of high cultivation requirements, restricting the development of the camellia market, and harsh environmental requirements, achieving high sensitivity, good effect, time-saving effect

Active Publication Date: 2021-10-22
PALM ECO TOWN DEV CO LTD +2
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  • Claims
  • Application Information

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Problems solved by technology

According to the statistics of the International Camellia Association, there are more than 20,000 varieties of camellias, and there are only more than 800 in my country. At present, most of the common camellias in the domestic market are imported from abroad. High, greatly restricting the development of the camellia market

Method used

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  • A Marking Method for Marking the Molecular ID Card of New Hybrid Varieties of Four Seasons Camellia
  • A Marking Method for Marking the Molecular ID Card of New Hybrid Varieties of Four Seasons Camellia
  • A Marking Method for Marking the Molecular ID Card of New Hybrid Varieties of Four Seasons Camellia

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Embodiment

[0026] Embodiment: Take 21 new hybrid varieties of Camellia japonica for four seasons as examples to further illustrate the present invention. Table 1: Information on 21 new hybrid varieties of Camellia japonica used in four seasons

[0027]

[0028] The method for marking the molecular identity card of the hybrid new variety of Four Seasons Camellia comprises the following steps:

[0029] 1. Extract the genomic DNA of 21 new hybrid varieties of Camellia japonica in Table 1;

[0030] 2. Perform SSR site screening, primer design and polymorphism detection of each SSR site:

[0031]a. The SSR locus was developed from the transcriptome data of the hybrid parent Dujuan Red Camellia, the RNA of Rhododendron Red Camellia was extracted, and the obtained RNA samples were sequenced. After obtaining the RNA-seq data, MISA software was used to scan the sequences The SSR locus, wherein, MISA search parameter setting: the repeating number of dinucleotide repeating unit ≥ 6; the repeat...

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Abstract

The invention discloses a marking method for calibrating a molecular identity card of a new hybrid variety of Camellia japonica, comprising the steps of: extracting the genomic DNA of the new variety to be used; performing SSR site screening, primer design and polymorphism detection of each SSR site; The 5' end of the forward primer of each SSR site is added with the M13 linker sequence that can be combined with the fluorescent label, the reverse primer sequence is unchanged, and the primer is synthesized, and the DNA sample is PCR amplified and sequenced. Data, genotype; convert these genotype data into numbers or letters as the molecular ID of each variety, and use the molecular ID to demarcate each camellia variety. The invention applies the fluorescent labeling SSR technology and the molecular identity card construction technology to the identification work of new camellia varieties, can accurately, reliably and efficiently identify different varieties, and construct a unique molecular identity card for each variety.

Description

【Technical field】 [0001] The invention relates to a marking method for marking a molecular ID card of a new hybrid variety of Four Seasons Camellia, which belongs to the field of molecular biology. 【Background technique】 [0002] Camellia is a traditional famous flower in my country. It is deeply loved by the public because of its green leaves and bright flowers. According to the statistics of the International Camellia Association, there are more than 20,000 varieties of camellias, and there are only more than 800 in my country. At present, most of the common camellias in the domestic market are imported from abroad. High, greatly restricting the development of the camellia market. The new variety of Four Seasons Camellia is a new generation of camellia varieties cultivated by Palm Ecological Town Development Co., Ltd. It has the advantages of bright colors, rich flower shapes, robust growth, strong stress resistance, and extensive management, and has great potential for ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/686
CPCC12Q1/686C12Q1/6895C12Q2600/156C12Q2525/151C12Q2563/107
Inventor 刘信凯张文驹陶乃奇严丹峰钟乃盛叶土生
Owner PALM ECO TOWN DEV CO LTD
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